Publications associées à Myogenèse

Inhibition of CERS1 in skeletal muscle exacerbates age-related muscle dysfunction

Johan Auwerx, Olivier Burri, Xiaoxu Li, Tanes Imamura de Lima, Giacomo Vincenzo Giorgio Von Alvensleben, Martin Rainer Wohlwend, Pirkka-Pekka Untamo Laurila, Ludger Jan Elzuë Goeminne, Amélia Lalou, Barbara Moreira Crisol, Renata Mangione

Age-related muscle wasting and dysfunction render the elderly population vulnerable and incapacitated, while underlying mechanisms are poorly understood. Here, we implicate the CERS1 enzyme of the de novo sphingolipid synthesis pathway in the pathogenesis ...

2023

In vivo transcriptomic profiling using cell encapsulation identifies effector pathways of systemic aging

Patrick Descombes, Jérome Feige, Sylviane Métairon, Omid Mashinchian, Filippo De Franceschi, Nicolas Bouche, Christophe Boss

Sustained exposure to a young systemic environment rejuvenates aged organisms and promotes cellular function. However, due to the intrinsic complexity of tissues it remains challenging to pinpoint niche-independent effects of circulating factors on specifi ...

2022

Molecular Regulation of Muscle Stem Cell Fate during Skeletal Muscle Regeneration and Aging

Sara Ancel

Muscle stem cells (MuSCs) are the primary source of myogenic progenitors during muscle repair and are essential for the long-term regenerative capacity of skeletal muscle. Following myofiber injury, MuSCs transition from a quiescent to an activated state, ...

EPFL2022

A Tead1-Apelin axis directs paracrine communication from myogenic to endothelial cells in skeletal muscle

Bart Deplancke, Julie Marie Russeil, Sonia Karaz, Maria Deak, Umji Lee, Benjamin D. Cosgrove

Apelin (Apln) is a myokine that regulates skeletal muscle plasticity and meta-bolism and declines during aging. Through a yeast one-hybrid transcription factor binding screen, we identified the TEA domain transcription factor 1 (Tead1) as a novel regulator ...

CELL PRESS2022

Deconstructing body axis morphogenesis in zebrafish embryos using robot-assisted tissue micromanipulation

Andrew Charles Oates, Mahmut Selman Sakar, Ece Özelçi, Erik Mailand, Matthias Rüegg

Classic microsurgical techniques, such as those used in the early 1900s by Mangold and Spemann, have been instrumental in advancing our understanding of embryonic development. However, these techniques are highly specialized, leading to issues of inter-ope ...

Nature Research2022

Products and methods for promoting myogenesis

Johan Auwerx, Martin Rainer Wohlwend

The present invention relates generally to the field of long non-coding RNAs and transcription factors and modulation of their expression for use in medicine and agriculture, such as the treatment and prevention of diseases associated with muscle atrophy a ...

2022

Investigation of the specificity and mechanism of action of the ULK1/AMPK inhibitor SBI-0206965

Kei Sakamoto, Katyayanee Neopane

SBI-0206965, originally identified as an inhibitor of the autophagy initiator kinase ULK1, has recently been reported as a more potent and selective AMP-activated protein kinase (AMPK) inhibitor relative to the widely used, but promiscuous inhibitor Compou ...

2021

Co-regulation of endothelial and myogenic cell fates through intercellular crosstalk in skeletal muscle

Umji Lee

Skeletal muscle is one of the most dynamic organs in the human body, which has a vigorous potential to regenerate and adapt to environmental changes. Depending on environments, skeletal muscle enables essential life activities to survive by controlling mov ...

EPFL2021

Notch signaling promotes disease initiation and progression in murine chronic lymphocytic leukemia

Freddy Radtke, Ute Koch, Jessica Sordet, Nadine Fournier, Marianne Nkosi, Delphine Maude Tardivon, Mateusz Waldemar Antoszewski

NOTCH1 gain-of-function mutations are recurrent in B-cell chronic lymphocytic leukemia (B-CLL), where they are associated with accelerated disease progression and refractoriness to chemotherapy. The specific role of NOTCH1 in the development and progressio ...

AMER SOC HEMATOLOGY2021

The Transcription Factor Nfix Requires RhoA-ROCK1 Dependent Phagocytosis to Mediate Macrophage Skewing during Skeletal Muscle Regeneration

Giuliana Rossi

Macrophages (MPs) are immune cells which are crucial for tissue repair. In skeletal muscle regeneration, pro-inflammatory cells first infiltrate to promote myogenic cell proliferation, then they switch into an anti-inflammatory phenotype to sustain myogeni ...

2020

Efficient decellularization of equine tendon with preserved biomechanical properties and cytocompatibility for human tendon surgery indications

Dominique Pioletti, Nathalie Hirt-Burri, Lee Ann Laurent-Applegate, Jens Antons, Cédric Peneveyre, Pierre-Arnaud Aeberhard, Anthony Grognuz

Chronic and acute tendon injuries are frequent afflictions, for which treatment is often long and unsatisfactory. When facing extended injuries, matrices and scaffolds with sufficient biomechanical properties are required for surgical repair and could addi ...

2020

Bioengineering the Human Muscle Stem-Cell Niche for Therapeutic Applications

Omid Mashinchian

In spite of decades of research, no feasible method for obtaining sufficient numbers of uncommitted muscle stem cells (MuSCs) for therapy of degenerative muscle diseases exists. One of the most fundamental problems associated with stem cell therapy of musc ...

EPFL2019

Podosomes, But not the Maturation status, Determine the Protease-Dependent 3D Migration in human Dendritic cells

Romain Guiet

Dendritic cells (DC) are professional Antigen-Presenting Cells scattered throughout antigen-exposed tissues and draining lymph nodes, and survey the body for pathogens. Their ability to migrate through tissues, a 3D environment, is essential for an effecti ...

2018

Heterogeneous combinatorial expression of Hoxd genes in single cells during limb development

Denis Duboule, Marion Leleu, Pierre Raymond Fabre

Background: Global analyses of gene expression during development reveal specific transcription patterns associated with the emergence of various cell types, tissues, and organs. These heterogeneous patterns are instrumental to ensure the proper formation ...

2018

Efficient transdifferentiation of human dermal fibroblasts into skeletal muscle

Noaf Salah Ali Alwahab

Skeletal muscle holds significant regenerative potential but is incapable of restoring tissue loss caused by severe injury, congenital defects or tumour ablation. Consequently, skeletal muscle models are being developed to study human pathophysiology and r ...

WILEY2018

An unbiased silencing screen in muscle cells identifies miR-320a, miR-150, miR-196b, and miR-34c as regulators of skeletal muscle mitochondria! metabolism

Johan Auwerx, Pénélope Andreux, Xu Wang

Objective: Strategies improving skeletal muscle mitochondrial capacity are commonly paralleled by improvements in (metabolic) health. We and others previously identified microRNAs regulating mitochondrial oxidative capacity, but data in skeletal muscle are ...

Elsevier Science Bv2017

Metabolic control of muscle and muscle stem cell function

Hongbo Zhang

Skeletal muscle composed of myofibers and a small amount of muscle stem cells (MuSCs). It plays important roles in energy metabolism. Some of the key metabolites, such as NAD+ and acetyl-CoA were recently found to regulate muscle and MuSCs function. Most o ...

EPFL2016

A KAP1 phosphorylation switch controls MyoD function during skeletal muscle differentiation

Didier Trono, Marco Cassano, Evaristo Jose Planet Letschert, Suk Min Jang

The transcriptional activator MyoD serves as a master controller of myogenesis. Often in partnership with Mef2 (myocyte enhancer factor 2), MyoD binds to the promoters of hundreds of muscle genes in proliferating myoblasts yet activates these targets only ...

Cold Spring Harbor Lab Press, Publications Dept2015

Myogenic potential of canine craniofacial satellite cells

Marco Cassano

The skeletal fibers have different embryological origin; the extraocular and jaw-closer muscles develop from prechordal mesoderm while the limb and trunk muscles from somites. These different origins characterize also the adult muscle stem cells, known as ...

Frontiers Research Foundation2014

A KRAB/KAP1-miRNA Cascade Regulates Erythropoiesis Through Stage-Specific Control of Mitophagy

Didier Trono, Jiri Vanicek, Sandra Eloise Kjeldsen, Isabelle Barde, Sonia Verp, Adamantia Kapopoulou, Andrea Corsinotti, Benjamin Rauwel, Ray Marcel Marin Florez

During hematopoiesis, lineage- and stage-specific transcription factors work in concert with chromatin modifiers to direct the differentiation of all blood cells. Here, we explored the role of KRAB-containing zinc finger proteins (KRAB-ZFPs) and their cofa ...

American Association for the Advancement of Science2013