Publications de Nicolas Pavillon

A Practical Inverse-Problem Approach to Digital Holographic Reconstruction

Christian Depeursinge, Michaël Unser, Nicolas Pavillon, Aurélien Bourquard, Emrah Bostan

In this paper, we propose a new technique for high-quality reconstruction from single digital holographic acquisitions. The unknown complex object field is found as the solution of a nonlinear inverse problem that consists in the minimization of an energy ...

OSA2013

Apparatus and method for quantitative phase tomography through linear scanning with coherent and non-coherent detection

Christian Depeursinge, Nicolas Pavillon

The disclosed invention describes a new apparatus performing a new data acquisition for quantitative refractive index tomography. It is based on a linear scanning of the specimen, opposed to the classical approaches based on rotations of either the sample ...

2013

Marker-free phase nanoscopy

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Nicolas Pavillon, Pascal Jourdain, Daniel Boss, Yann Jérôme Michel Pascal Cotte, Muhammed Fatih Toy

We introduce a microscopic method that determines quantitative optical properties beyond the optical diffraction limit and allows direct imaging of unstained living biological specimens. In established holographic microscopy, complex fields are measured us ...

Nature Publishing Group2013

Single-shot, simultaneous incoherent and holographic microscopy

Christian Depeursinge, Nicolas Pavillon, Etienne Shaffer

In this work, we demonstrate single-shot, simultaneous recording and subsequent retrieval of one incoherent and two holographic (intensity and phase) images from the same camera frame. Demultiplexing of incoherent and holographic signals in the spatial fre ...

Wiley-Blackwell2012

Early Cell Death Detection with Digital Holographic Microscopy

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Nicolas Pavillon, Pascal Jourdain, Corinne Moratal

Background: Digital holography provides a non-invasive measurement of the quantitative phase shifts induced by cells in culture, which can be related to cell volume changes. It has been shown previously that regulation of cell volume, in particular as it r ...

Public Library of Science2012

Local demodulation of holograms using the Riesz transform with application to microscopy

Christian Depeursinge, Michaël Unser, Nicolas Pavillon, Chandra Sekhar Seelamantula

We propose a Riesz transform approach to the demodulation of digital holograms. The Riesz transform is a higher-dimensional extension of the Hilbert transform and is steerable to a desired orientation. Accurate demodulation of the hologram requires a relia ...

Optical Society of America2012

Exact Complex-Wave Reconstruction in Digital Holography

Christian Depeursinge, Michaël Unser, Nicolas Pavillon, Chandra Sekhar Seelamantula

We address the problem of exact complex-wave reconstruction in digital holography. We show that, by confining the object-wave modulation to one quadrant of the frequency domain, and by maintaining a reference-wave intensity higher than that of the object, ...

OSA2011

Cellular Dynamics and Three-Dimensional Refractive Index Distribution Studied with Quantitative Phase Imaging

Nicolas Pavillon

We present in this PhD thesis work various applications of digital holographic microscopy (DHM), an imaging technique based on coherent illumination which enables the recovery of the full complex wavefront, i.e. the amplitude and phase of a wave field whic ...

EPFL2011

Complex index refraction tomography with sub ?/6-resolution

Christian Depeursinge, Nicolas Pavillon, Yann Jérôme Michel Pascal Cotte

The present invention discloses a method to improve the image resolution of a microscope. This improvement is based on the mathematical processing of the complex field computed from the measurements with a microscope of the wave emitted or scattered by the ...

2011

Determination of Transmembrane Water Fluxes in Neurons Elicited by Glutamate Ionotropic Receptors and by the Co-transporters KCC2 and NKCC1: A Digital Holographic Microscopy Study

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Nicolas Pavillon, Pascal Jourdain, Daniel Boss, Benjamin Rappaz, Corinne Moratal

Digital holographic microscopy (DHM) is a noninvasive optical imaging technique that provides quantitative phase images of living cells. In a recent study, we showed that the quantitative monitoring of the phase signal by DHM was a simple label-free method ...

Society for Neuroscience2011

Digital holographic reflectometry

Tristan Colomb, Christian Depeursinge, Jonas Kühn, Nicolas Pavillon, Ahmet Ata Akatay

Digital holographic microscopy (DHM) is an interferometric technique that allows real-time imaging of the entire complex optical wavefront (amplitude and phase) reflected by or transmitted through a sample. To our knowledge, only the quantitative phase is ...

2010

Microscopy image resolution improvement by deconvolution of complex fields

Christian Depeursinge, Nicolas Pavillon, Yann Jérôme Michel Pascal Cotte, Muhammed Fatih Toy

Based on truncated inverse filtering, a theory for deconvolution of complex fields is studied. The validity of the theory is verified by comparing with experimental data from digital holographic microscopy (DHM) using a high-NA system (NA=0.95). Comparison ...

Optical Society of America2010

Sub-Rayleigh resolution by phase imaging

Christian Depeursinge, Nicolas Pavillon, Etienne Shaffer, Yann Jérôme Michel Pascal Cotte, Muhammed Fatih Toy

We report the experimental observation of systematically occurring phase singularities in coherent imaging of sub-Rayleigh distanced objects. A theory that relates the observation to the sub-Rayleigh distance is presented and compared with experimental mea ...

Optical Society of America2010

Endoscopic low coherence topography measurement for upper airways and hollow samples

Christian Depeursinge, Jonas Kühn, Yves Delacrétaz, Nicolas Pavillon, Etienne Shaffer, Florian Lang

To evaluate the severity of airway pathologies, quantitative dimensioning of airways is of utmost importance. Endoscopic vision gives a projective image and thus no true scaling information can be directly deduced from it. In this article, an approach base ...

SPIE Society of Photo-optical Instrumentation Engineers2010

A method and apparatus for enhanced spatial bandwidth wavefronts reconstructed from digital interferograms or holograms

Christian Depeursinge, Michaël Unser, Nicolas Pavillon, Chandra Sekhar Seelamantula

The present invention discloses a method and an apparatus to compute a complex wavefield, referred to as the object wave o, by means of measuring the intensity signal resulting from the interference of the said object wave with a second wave termed the ref ...

2010

Cell Morphology and Intracellular Ionic Homeostasis explored with a Multimodal Approach combining Epifluorescence and Digital Holographic Microscopy

Christian Depeursinge, Pierre Marquet, Jonas Kühn, Pierre Magistretti, Nicolas Pavillon, Pascal Jourdain, Daniel Boss, Corinne Moratal, Aleksandr Benke

The authors have developed a live-cell multimodality microscope combining epifluorescence with digital holographic microscopy; it has been implemented with a decoupling procedure allowing to separately measure from the quantitative phase important cell par ...

2010

Iterative method for zero-order suppression in off-axis digital holography

Christian Depeursinge, Nicolas Pavillon, Cristian Arfire

We propose a method to suppress the so-called zero-order term in a hologram, based on an iterative principle. During the hologram acquisition process, the encoded information includes the intensities of the two beams creating the interference pattern, whic ...

Optical Society of America2010

Extended depth-of-focus by digital holographic microscopy

Tristan Colomb, Etienne Cuche, Christian Depeursinge, Jonas Kühn, Nicolas Pavillon

A recurrent problem in microscopy is the finite depth-of-focus linked to the NA of microscope objectives. Digital holographic microscopy (DHM) has the unique feature of being able to numerically change the focus from a single hologram without the need of m ...

Optical Society of America2010

Zero-Order-Free Image Reconstruction in Digital Holographic Microscopy

Christian Depeursinge, Michaël Unser, Nicolas Pavillon, Chandra Sekhar Seelamantula

We address the problem of zero-order-free image reconstruction in digital holographic microscopy. We show how the goal can be achieved by confining the object-wave modulation to one quadrant of the frequency domain, and by maintaining a reference-wave inte ...

IEEE2009

A Nonlinear Technique for Automatic Twin-Image and Zero-Order Term Suppression in Digital Holographic Microscopy

Christian Depeursinge, Michaël Unser, Nicolas Pavillon, Chandra Sekhar Seelamantula

ITO2009