Publications de Cédric Deluz | EPFL Graph Search

Orchestration of pluripotent stem cell genome reactivation during mitotic exit

Cédric Deluz, David Michael Suter, Silja Michelle Placzek, Ludovica Vanzan

Cell identity maintenance faces many challenges during mitosis, as most DNA-binding proteins are evicted from DNA and transcription is virtually abolished. How cells maintain their identity through division and faithfully re-initiate gene expression during ...

2025

Endogenous fluctuations of OCT 4 and SOX 2 bias pluripotent cell fate decisions

Cédric Deluz, David Michael Suter, Daniel Strebinger, Andrea Brigitta Alber, Torgil Elias Friman, Subashika Govindan

SOX2 and OCT4 are pioneer transcription factors playing a key role in embryonic stem (ES) cell self‐renewal and differentiation. How temporal fluctuations in their expression levels bias lineage commitment is unknown. Here, we generated knock‐in reporter f ...

2019

Mitotic chromosome binding predicts transcription factor properties in interphase

Cédric Deluz, Mahé Cécile Raccaud, David Michael Suter, Andrea Brigitta Alber, Torgil Elias Friman

Mammalian transcription factors (TFs) differ broadly in their nuclear mobility and sequence-specific/non-specific DNA binding. How these properties affect their ability to occupy specific genomic sites and modify the epigenetic landscape is unclear. The as ...

Nature Research2019

Dynamic regulation of chromatin accessibility by pluripotency transcription factors across the cell cycle

Cédric Deluz, Bart Deplancke, David Michael Suter, Antonio Carlos Alves Meireles Filho, Torgil Elias Friman, Vincent Roland Julien Gardeux, Subashika Govindan

The pioneer activity of transcription factors allows for opening of inaccessible regulatory elements and has been extensively studied in the context of cellular differentiation and reprogramming. In contrast, the function of pioneer activity in self-renewi ...

2019

Engineered Multivalent Sensors to Detect Coexisting Histone Modifications in Living Stem Cells

Horst Pick, Cédric Deluz, Bart Deplancke, Aurore Marie-France Delachat, Nora Guidotti, Beat Fierz, David Michael Suter, Antonio Carlos Alves Meireles Filho, Carolin Christine Lechner, Andreas Linus Bachmann

2018

Mitotic chromosome association predicts genome-wide transcription factor occupancy and impact on local chromatin accessibility

Cédric Deluz, Mahé Cécile Raccaud, David Michael Suter, Andrea Brigitta Alber, Torgil Elias Friman

MARY ANN LIEBERT, INC2018

Endogenous fluctuations of OCT4 and SOX2 bias pluripotent cell fate decisions

Cédric Deluz, David Michael Suter, Daniel Strebinger, Andrea Brigitta Alber, Torgil Elias Friman, Subashika Govindan

MARY ANN LIEBERT, INC2018

OCT4 and SOX2 are required at the M-G1 transition to re-establish chromatin accessibility at enhancers in pluripotent stem cells

Cédric Deluz, David Michael Suter, Antonio Carlos Alves Meireles Filho, Torgil Elias Friman, Vincent Roland Julien Gardeux

MARY ANN LIEBERT, INC2018

A role for mitotic bookmarking of SOX2 in pluripotency and differentiation

Cédric Deluz, Mahé Cécile Raccaud, Suliana Manley, Marion Leleu, Aleksandr Benke, Andrea Callegari, David Michael Suter, Daniel Strebinger, Torgil Elias Friman

Mitotic bookmarking transcription factors remain bound to chromosomes during mitosis and were proposed to regulate phenotypic maintenance of stem and progenitor cells at the mitosis-to-G1 (M-G1) transition. However, mitotic bookmarking remains largely unex ...

Cold Spring Harbor Laboratory2016

The Structure of the Mouse Serotonin 5-HT3 Receptor in Lipid Vesicles

Henning Paul-Julius Stahlberg, Horst Vogel, Cédric Deluz, Luigino Grasso, Ghérici Hassaïne

The function of membrane proteins is best understood if their structure in the lipid membrane is known. Here, we determined the structure of the mouse serotonin 5-HT3 receptor inserted in lipid bilayers to a resolution of 12 Å without stabilizing antibodie ...

Elsevier2016

X-ray structure of the mouse serotonin 5-HT3 receptor

Rudolf Hovius, Henning Paul-Julius Stahlberg, Horst Vogel, Cédric Deluz, Luigino Grasso, Romain Wyss, Menno Berend Tol, Ghérici Hassaïne

Neurotransmitter-gated ion channels of the Cys-loop receptor family mediate fast neurotransmission throughout the nervous system. The molecular processes of neurotransmitter binding, subsequent opening of the ion channel and ion permeation remain poorly un ...

Nature Publishing Group2014

Large scale expression and purification of the mouse 5-HT3 receptor (vol 1828, pg 2544, 2013)

Horst Vogel, Cédric Deluz, Menno Berend Tol, Ghérici Hassaïne

Elsevier Science Bv2014

Thermal Unfolding of a Mammalian Pentameric Ligand-gated Ion Channel Proceeds at Consecutive, Distinct Steps

Henning Paul-Julius Stahlberg, Horst Vogel, Cédric Deluz, Menno Berend Tol, Ghérici Hassaïne

Pentameric ligand-gated ion channels (LGICs) play an important role in fast synaptic signal transduction. Binding of agonists to the beta-sheet-structured extracellular domain opens an ion channel in the transmembrane alpha-helical region of the LGIC. How ...

Amer Soc Biochemistry Molecular Biology Inc2013

Large scale expression and purification of the mouse 5-HT3 receptor

Horst Vogel, Cédric Deluz, Menno Berend Tol, Ghérici Hassaïne

Receptors of the Cys-loop family are central to neurotransmission and primary therapeutic targets. In order to decipher their gating and modulation mechanisms, structural data is essential. However, structural studies require large amounts of pure, functio ...

Elsevier Science Bv2013

A Zeptoliter Volume Meter for Analysis of Single Protein Molecules

Horst Vogel, Christian Santschi, Cédric Deluz, Olivier Martin, Romain Wyss, Ghérici Hassaïne

American Chemical Society (ACS)2012

Ligand binding transmits conformational changes across the membrane-spanning region to the intracellular side of the 5-HT3 serotonin receptor

Horst Vogel, Horst Pick, Erwin Ilegems, Cédric Deluz

Conformational changes of channel activation: Five enhanced green fluorescent protein (EGFP) mols. (green cylinders) were integrated into the intracellular part of the homopentameric ionotropic 5-HT3 receptor. This allowed the detection of extracellular bi ...

2005