Publications de Christopher Chidley

Compositions and use of sulfasalazine

The invention relates to compositions comprising sulfasalazine or sulfapyridine and products of a biosynthetic pathway that requires tetrahydrobiopterin. Such compositions are useful for the treatment of inflammatory bowel diseases or rheumatoid arthritis, ...

2011

A yeast-based screen reveals that sulfasalazine inhibits tetrahydrobiopterin biosynthesis

Kai Johnsson, Christopher Chidley, Evelyne Muller, Miriam Grønlund Pedersen, Hirohito Haruki

2011

Searching for the Protein Targets of Bioactive Molecules

Kai Johnsson, Christopher Chidley, Cindy Fellay, Miriam Grønlund Pedersen, Simone Moser, Hirohito Haruki

The identification of all protein targets of a given drug or bioactive molecule within the human body is a prerequisite for an understanding of its beneficial and deleterious activities. Current approaches to reveal protein targets often fail to reveal phy ...

Schweizerische Chemische Gesellschaft2011

A Novel Yeast-Based Platform for Small Molecule Target Deconvolution Reveals Previously Unknown Binding Partners for the Approved Drugs Erlotinib, Atorvastatin, and Sulfasalazine

Christopher Chidley

The identification of all protein targets of a small molecule drug, i.e. target deconvolution, provides the basis for understanding its beneficial or deleterious actions. Target deconvolution remains a major and often problematic task within the biotechnol ...

EPFL2010

A designed protein for the specific and covalent hetero-conjugation of biomolecules

Kai Johnsson, Christopher Chidley, Katarzyna Mosiewicz

2008

Method of crosslinking two objects of interest

Kai Johnsson, Christopher Chidley, Katarzyna Mosiewicz

The invention provides a method of crosslinking two objects of interest, comprising the steps of : i) providing a fusion protein comprising at least a first protein and a second protein, wherein both the first and the second protein are, based on their str ...

2008

Mutants of o6-alkylguanine-dna alkyltransferase

Kai Johnsson, Christopher Chidley, Antje Keppler, Alexandre Juillerat, Thomas Gronemeyer, Christian Heinis

The invention relates to AGT mutants showing, when compared to the wild type human AGT, two or more advantageous properties selected from (a) reduced DNA interaction (b) localisation of the expressed protein in eukaryotic cells that is no longer restricted ...

2006

Directed evolution of O6-alkylguanine-DNA alkyltransferase for applications in protein labeling

Kai Johnsson, Christopher Chidley, Alexandre Juillerat, Thomas Gronemeyer, Christian Heinis

The specific reaction of O6-alkylguanine-DNA alkyltransferase (AGT) with O6-benzylguanine (BG) derivatives allows for a specific labeling of AGT fusion proteins with chemically diverse compounds in living cells and in vitro. The efficiency of the labeling ...

Oxford University Press2006