Publications de Paolo Silacci | EPFL Graph Search

Microfluidic chip for monitoring Ca2+ transport through a confluent layer of intestinal cells

Martinus Gijs, Paolo Silacci, Guy Vergères, Hüseyin Cumhur Tekin, Josias Basil Wacker, Christine Ruffert

The absorption of dietary calcium through the intestinal barrier is essential for maintaining health in general and especially of the bone system. We propose a microfluidic model that studies free calcium (Ca2+) transport through a confluent monolayer of C ...

Royal Soc Chemistry2014

Quantitative Estimation of Biological Cell Surface Receptors by Segmenting Conventional Fluorescence Microscopy Images

Paolo Silacci, Guy Vergères, Giovanni De Micheli, Julien Michel Ghaye, Sandro Carrara, Chiara Succa, Danilo Demarchi

State-of-the-art techniques for measuring and monitoring gene level expression rely on messenger RNA (mRNA) extraction and quantification, usually based on the concept of reverse transcription polymerase chain reaction. In this paper, we take adva ...

Ieee2014

NutriChip: Nutrition Analysis meets Microfluidics

Martinus Gijs, Paolo Silacci, Guy Vergères, Gözen Köklü, Julien Michel Ghaye, Sandro Carrara, Hamideh Jafarpoorchekab, Christine Ruffert

This focus article introduces the concept of NutriChip, an integrated microfluidic platform for investigating the potential of the immuno-modulatory function of dairy food. The core component of the NutriChip is a miniaturized artificial human gastrointest ...

Royal Society of Chemistry2013

Image thresholding techniques for localization of sub-resolution fluorescent biomarkers

Paolo Silacci, Guy Vergères, Giovanni De Micheli, Julien Michel Ghaye, Sandro Carrara, Madhura Avinash Kamat

In this article, we explore adaptive global and local segmentation techniques for a lab-on-chip nutrition monitoring system (NutriChip). The experimental setup consists of Caco-2 intestinal cells that can be artificially stimulated to trigger an immune res ...

Wiley-Blackwell2013

The NutriChip project - translating technology into nutritional knowledge

Martinus Gijs, Paolo Silacci, Guy Vergères, Sandro Carrara, Flurina Schwander, Reto Portmann

Advances in food transformation have dramatically increased the diversity of products on the market and, consequently, exposed consumers to a complex spectrum of bioactive nutrients whose potential risks and benefits have mostly not been confidently demons ...

Cambridge University Press2012

NutriChip - A lab-on-a-chip platform to investigate food absorption and underlying immune regulations

Martinus Gijs, Paolo Silacci, Guy Vergères, Flurina Schwander, Reto Portmann

Federation Amer Soc Exp Biol2012

Gastrointestinal Tract on Chip

Martinus Gijs, Paolo Silacci, Guy Vergères, Hamideh Jafarpoorchekab

2012

Nutrichip: an integrated microfluidic system for in vitro investigation of the immunemodulatory function of dairy products

Martinus Gijs, Paolo Silacci, Guy Vergères, Sandro Carrara, Flurina Schwander, Hamideh Jafarpoorchekab

We introduce the concept of the NutriChip, a miniaturized artificial human gastrointestinal tract (GIT) for investigating the potential of immuno-modulatory function of dairy food. More specifically, we are investigating the effect of digested milk on immu ...

2011

Autonomous effects of shear stress and cyclic circumferential stretch regarding endothelial dysfunction and oxidative stress: an ex vivo arterial model

Nikolaos Stergiopoulos, Paolo Silacci, Tyler Nerton Thacher, Rafaela Fernandes Da Silva

Cyclic circumferential stretch and shear stress caused by pulsatile blood flow work in concert, yet are very different stimuli capable of independently mediating endothelial function by modulating eNOS expression, oxidative stress (via production of supero ...

American Soc Mechanical Engineers, Three Park Avenue, New York, Ny 10016-5990 Usa2009

Arterial biomechanics after destruction of cytoskeleton by Cytochalasin D

Nikolaos Stergiopoulos, Paolo Silacci, Sylvain Roy, Tyler Nerton Thacher

Vascular smooth muscle is a major structural element of the arterial wall. We examined the effects of cytoskeleton destruction, after administration of Cytochalasin D, on the biomechanical properties of porcine common carotids. Compared to untreated, maxim ...

2009

Reduced cyclic stretch, endothelial dysfunction, and oxidative stress: an ex vivo model

Nikolaos Stergiopoulos, Veronica Gambillara, Paolo Silacci, Tyler Nerton Thacher, Rafaela Fernandes Da Silva

The objective of this study was to investigate whether reduction of cyclic circumferential stretch will impair endothelial function and elevate basal levels of oxidative stress, both known risk factors linked to cardiovascular disease. ...

2009

Regulation of arginase pathway in response to wall shear stress

Nikolaos Stergiopoulos, Veronica Gambillara, Paolo Silacci, Fabienne Riche, Tyler Nerton Thacher, Rafaela Fernandes Da Silva

Alterations of wall shear stress can predispose the endothelium to the development of atherosclerotic plaques. Ample evidence indicates that arginase expression and/or activity correlates with several risk factors for cardiovascular disease including ather ...

2009

Effects Of Reduced Compliance On Endothelial Functionality With Regards To Inflammation

Nikolaos Stergiopoulos, Veronica Gambillara, Paolo Silacci, Tyler Nerton Thacher, Rafaela Fernandes Da Silva

American Soc Mechanical Engineers, Three Park Avenue, New York, Ny 10016-5990 Usa2009

Transcriptional and post-transcriptional regulation of endothelin-1 gene by shear stress

Nikolaos Stergiopoulos, Paolo Silacci, Rafaela Fernandes Da Silva

Objective Plaque-prone areas are exposed to a particular hemodynamic environment characterized by a low mean shear stress value and a cyclic reversal flow. This mechanical environment, also termed oscillatory shear stress (OSS), induces the expression of s ...

2008

Effects of reduced cyclic stretch on vascular smooth muscle cell function of pig carotids perfused ex vivo

Nikolaos Stergiopoulos, Veronica Gambillara, Paolo Silacci, Tyler Nerton Thacher

With advancing age arteries stiffen, reducing arterial compliance and leading to the development of systolic hypertension and to a substantial increase in pulse pressure. An augmented pulse pressure can be a predictor of the development of hypertension, wh ...

Oxford University Press2008

Endothelialization Of Decellularized Porcine Arteries

Nikolaos Stergiopoulos, Paolo Silacci, Sylvain Roy

2008

Oscillatory shear stress and reduced compliance impair vascular functions

Nikolaos Stergiopoulos, Veronica Gambillara, Paolo Silacci, Tyler Nerton Thacher, Rafaela Fernandes Da Silva

Shear stress has been shown to influence endothelial cell gene expression and morphology. In particular, low and bi-directional shear stress, mimicking conditions at plaque-prone areas, down-regulates the expression of several atheroprotective genes, and u ...

2007

Transcriptional and post-transcriptional regulation of preproendothelin-1 by plaque-prone hemodynamics

Nikolaos Stergiopoulos, Paolo Silacci, Rafaela Fernandes Da Silva

Plaque-prone areas are exposed to a particular hemodynamic environment characterized by a low mean shear stress value and a cyclic reversal flow. This mechanical environment, also termed oscillatory shear stress (OSS), induces the expression of several pro ...

2007

RGD-Functionalized polymer brushes as substrates for the integrin specific adhesion of human umbilical vein endothelial cells

Nikolaos Stergiopoulos, Paolo Silacci, Harm-Anton Klok, Stefano Tugulu

This report demonstrates the feasibility of surface-initiated atom transfer radical polymerization to prepare thin polymer layers ("brushes") that can be functionalized with short peptide ligands and which may be of use as coatings to promote endothelializ ...

2007

Shear stress and cyclic circumferential stretch, but not pressure, alter connexin43 expression in endothelial cells

Nikolaos Stergiopoulos, Paolo Silacci

Hemodynamic forces play a critical role in atherogenesis, as evidenced by the focal pattern of development of atherosclerotic lesions. Whereas disturbed flow in the branches and curved regions of large arteries is proatherogenic, laminar flow in the straig ...

2006