Publications de Steffen Cosson

Ultra-rapid prototyping of flexible, multi-layered microfluidic devices via razor writing

Matthias Lütolf, Steffen Cosson, Nathalie Brandenberg

The fabrication of microfluidic devices is often still a time-consuming and costly process. Here we introduce a very simple and cheap microfabrication process based on "razor writing", also termed xurography, for the ultra-rapid prototyping of microfluidic ...

Royal Soc Chemistry2015

Hydrogel microfluidics for the patterning of pluripotent stem cells

Matthias Lütolf, Steffen Cosson

Biomolecular signaling is of utmost importance in governing many biological processes such as the patterning of the developing embryo where biomolecules regulate key cell-fate decisions. In vivo, these factors are presented in a spatiotemporally tightly co ...

Nature Publishing Group2014

A high-capacity cell macroencapsulation system supporting the long-term survival of genetically engineered allogeneic cells

Patrick Aebischer, Matthias Lütolf, Bernard Schneider, Steffen Cosson, Aurélien Lathuilière

The rapid increase in the number of approved therapeutic proteins, including recombinant antibodies, for diseases necessitating chronic treatments raises the question of the overall costs imposed on healthcare systems. It is therefore important to investig ...

Elsevier2014

Microfluidic Patterning of Protein Gradients on Biomimetic Hydrogel Substrates

Matthias Lütolf, Steffen Cosson

This protocol describes a versatile microfluidic method to generate tethered protein gradients of virtually any user-defined shape on biomimetic hydrogel substrates. It can be applied to test, in a microenvironment of physiologically relevant stiffness, ho ...

Elsevier Academic Press Inc2014

Patterning of cell-instructive hydrogels by hydrodynamic flow focusing

Matthias Lütolf, Steffen Cosson, Simone Allazetta

Microfluidic gradient systems offer a very precise means to probe the response of cells to graded biomolecular signals in vitro, for example to model how morphogen proteins affect cell fate during developmental processes. However, existing gradient makers ...

Royal Society of Chemistry2013

Hydrogel Microfluidics to Control Stem Cell Fate

Steffen Cosson

Biomolecular signaling is of utmost importance in governing many biological processes such as morphogenesis during tissue development where biomolecules regulate key cell-fate decisions. In vivo, these factors are presented in a spatiotemporally tightly co ...

EPFL2012

Programmable microfluidic patterning of protein gradients on hydrogels

Matthias Lütolf, Steffen Cosson, Simone Allazetta

Computer-controlled hydrodynamic flow focusing was utilized to generate tethered protein gradients of any user-defined shape on the surface of soft synthetic hydrogels. ...

Royal Society of Chemistry2010

Capturing Complex Protein Gradients on Biomimetic Hydrogels for Cell-Based Assays

Matthias Lütolf, Steffen Cosson, Stefan Kobel

versatile strategy to rapidly immobilize complex gradients of virtually any desired protein on soft poly(ethylene glycol) (PEG) hydrogel surfaces that are reminiscent of natural extracellular matrices (ECM) is reported. A microfluidic chip is used to gener ...

2009

Microfluidics-generated immobilized biomolecule gradients on hydrogels

Matthias Lütolf, Steffen Cosson, Stefan Kobel

Extra-cellular matrix (ECM) tethered morphogen gradients play an important role in tissue development and regeneration. There is currently only a limited set of model systems which can mimic these gradients in an artificial ECM-like environment. We have de ...

The Chemical and Biological Microsystems Society2008