Publications de India Christina Sielaff

A Covalent Chemical Genotype-Phenotype Linkage for in vitro Protein Evolution

2007

Protein function microarrays based on self-immobilizing and self-labeling fusion proteins

Kai Johnsson, India Christina Sielaff, Harm-Anton Klok, Anke Arnold, Stefano Tugulu

Protein microarrays are an attractive approach for the high-throughput analysis of protein function, but their impact on proteomics has been limited by the technical difficulties associated with their generation. Here we demonstrate that fusion proteins of ...

2006

Specific substrates for O6- alkylguanine-DNA alkyltransferase

Kai Johnsson, India Christina Sielaff

The invention relates to substrates for O-alkylguanine-DNA alkyltransferases (AGT) of formula R1-A-X-CH2-R3-R4-L1, wherein A is a group recognized by AGT as a substrate, X is oxygen or sulfur, R1 is a group -R2-L2 or a group R5, R2 and R4 are, independe ...

2006

Chemical approaches to microarrays and multifunctional protein labeling

India Christina Sielaff

With the human genome and many other eukaryotic and procaryotic genomes sequenced, the interest in life sciences is shifting towards the examination of the function, modification and regulation of the encoded proteins. Proteins are involved in virtually al ...

EPFL2006

Protein-functionalized polymer brushes

Kai Johnsson, India Christina Sielaff, Harm-Anton Klok, Anke Arnold, Stefano Tugulu

Understanding and controlling the interactions between synthetic and biol. materials is of great importance for the development of novel materials for a variety of biomedical and bioanal. applications, including for example stents, vascular grafts and prot ...

2005

Engineering substrate specificity of O6-alkylguanine-DNA alkyltransferase for specific protein labeling in living cells

Hugues Jaccard, Kai Johnsson, Alexandre Juillerat, India Christina Sielaff, Béatrice Kunz, Christian Heinis

Fusion proteins of human O6-alkylguanine-DNA alkyltransferase (AGT) can be specifically labeled with a wide variety of synthetic probes in mammalian cells; this makes them an attractive tool for studying protein function. However, to avoid undesired labeli ...

2005

Protein-Functionalized Polymer Brushes

Kai Johnsson, India Christina Sielaff, Harm-Anton Klok, Anke Arnold, Stefano Tugulu

A new strategy for the prepn. of protein-functionalized polymer brushes is reported, which is based on a combination of surface-initiated atom transfer radical polymn. (ATRP), p-nitrophenyl chloroformate activation of the surface hydroxyl groups, and subse ...

American Chemical Society2005