Publications de Peter Frey | EPFL Graph Search

Off-the-Shelf Implant to Bridge a Urethral Defect: Multicenter 8-Year Journey From Bench to Bed

Objective: To engineer an acellular mesh to reconstruct the urethra to replace the current surgical practice of using autologous tissue grafts. Cell based approaches have shown progress. However, these have been associated with high costs and logistical ch ...

2024

A bioactive injectable bulking material; a potential therapeutic approach for stress urinary incontinence

Matthias Lütolf, Jeffrey Alan Hubbell, Peter Frey, Hans Mattias Larsson, Elif Vardar, Kalitha Pinnagoda, Ganesh Vythilingam

Stress urinary incontinence (SUI) is a life changing condition, affecting 20 million women worldwide. In this study, we developed a bioactive, injectable bulking agent that consists of Permacol (TM) (Medtronic, Switzerland) and recombinant insulin like gro ...

ELSEVIER SCI LTD2019

Quantitative intrinsic auto-cathodoluminescence can resolve spectral signatures of tissue-isolated collagen extracellular matrix

Jeffrey Alan Hubbell, Peter Frey, Hans Mattias Larsson, Elif Vardar, Marcin Stefan Zielinski, Ganesh Vythilingam

By analyzing isolated collagen gel samples, we demonstrated in situ detection of spectrally deconvoluted auto-cathodoluminescence signatures of specific molecular content with precise spatial localization over a maximum field of view of 300 mu m. Correlati ...

2019

Fiber density of collagen grafts impacts rabbit urethral regeneration

Jeffrey Alan Hubbell, Peter Frey, Hans Mattias Larsson, Elif Vardar, Kalitha Pinnagoda, Ganesh Vythilingam

There is a need for efficient and “off-the-shelf” grafts in urethral reconstructive surgery. Currently available surgical techniques require harvesting of grafts from autologous sites, with increased risk of surgical complications and added patient discomf ...

Nature Research2018

Microfluidic production of bioactive fibrin micro-beads embedded in crosslinked collagen used as an injectable bulking agent for urinary incontinence treatment

Matthias Lütolf, Jeffrey Alan Hubbell, Peter Frey, Hans Mattias Larsson, Simone Allazetta, Elif Vardar, Kalitha Pinnagoda, Ganesh Vythilingam

2018

Latest Progress in Tissue Engineered Urethral Regeneration. From Rabbit to Dog, A Step from Human Clinical Trial for Surgical Treatment of VSD (Variation of Sex Development)

Peter Frey, Hans Mattias Larsson, Elif Vardar, Kalitha Pinnagoda, Ganesh Vythilingam

KARGER2018

Human Kunitz-type protease inhibitor engineered for enhanced matrix retention extends longevity of fibrin biomaterials

Jeffrey Alan Hubbell, Peter Frey, Kristen Lorentz, Priscilla Suhasna Maithili Briquez, Hans Mattias Larsson

Aprotinin is a broad-spectrum serine protease inhibitor used in the clinic as an anti-fibrinolytic agent in fibrin-based tissue sealants. However, upon re-exposure, some patients suffer from hypersensitivity immune reactions likely related to the bovine or ...

2017

Natural polymer-derived scaffold material and methods for production thereof

Matthias Lütolf, Jeffrey Alan Hubbell, Peter Frey, Eva-Maria Balet, Hans Mattias Larsson, Simone Allazetta, Elif Vardar, Kalitha Pinnagoda

The invention relates to a scaffold material comprising a carrier and embedded microbeads for use in tissue engineering applications such as soft tissues therapeutic treatment. The scaffold provides a short-term bulking effect coupled with a long-term func ...

2017

Engineered acellular collagen scaffold for endogenous cell guidance, a novel approach in urethral regeneration

Jeffrey Alan Hubbell, Peter Frey, Hans Mattias Larsson, Elif Vardar, Kalitha Pinnagoda, Ganesh Vythilingam

The treatment of congenital malformations or injuries of the urethra using existing autologous tissues can be associated with post-operative complications. Using rat-tail collagen, we have engineered an acellular high-density collagen tube. These tubes wer ...

Elsevier Sci Ltd2016

IGF-1-containing multi-layered collagen-fibrin hybrid scaffolds for bladder tissue engineering

Jeffrey Alan Hubbell, Peter Frey, Priscilla Suhasna Maithili Briquez, Hans Mattias Larsson, Elif Vardar, Kalitha Pinnagoda

Clinical success of bladder reconstructive procedures could be promoted by the availability of functional biomaterials. In this study, we have developed a multi-layered scaffold consisting of a bioactive fibrin layer laminated between two collagen sheets a ...

Elsevier Sci Ltd2016

Tissue Engineered Collagen Based Tubular Scaffolds for Urethral Regeneration. A Novel Technology for the Surgical Treatment of VSD (Variation of Sex Development) Patients with Severe Hypospadias

Jeffrey Alan Hubbell, Peter Frey, Hans Mattias Larsson, Elif Vardar, Kalitha Pinnagoda, Ganesh Vythilingam

Karger2016

Tubular Compressed Collagen Scaffolds for Ureteral Tissue Engineering in a Flow Bioreactor System

Jeffrey Alan Hubbell, Peter Frey, Hans Mattias Larsson, Elif Vardar, Mouti Elodie Mouloungui, Kalitha Pinnagoda

Ureteral replacement by tissue engineering might become necessary following tissue loss after excessive ureteral trauma, after retroperitoneal cancer, or even after failed reconstructive surgery. This need has driven innovation in the design of novel scaff ...

Mary Ann Liebert, Inc2015

Collagen Based Tubular Structures for Urethral Repair in a Rabbit Model

Jeffrey Alan Hubbell, Peter Frey, Eva-Maria Balet, Hans Mattias Larsson, Elif Vardar, Kalitha Pinnagoda, Ganesh Vythilingam

Mary Ann Liebert, Inc2015

Off-the Shelf Collagen-fibrin based Scaffolds for Bladder Augmentation

Jeffrey Alan Hubbell, Peter Frey, Eva-Maria Balet, Hans Mattias Larsson, Elif Vardar, Kalitha Pinnagoda

Mary Ann Liebert, Inc2015

Is There a Need for Smooth Muscle Cell Transplantation in Urethral Reconstruction?

Peter Frey

Background: Hypospadias and urethral strictures are conditions requiring additional tissue for reconstruction. Due to a limited source of tissue, autologous skin and oral mucosa are frequently used. However, long-term follow-up studies demonstrated signifi ...

Mary Ann Liebert, Inc2014

Clonal, Self-Renewing and Differentiating Human and Porcine Urothelial Cells, a Novel Stem Cell Population

Jeffrey Alan Hubbell, Yann Barrandon, Peter Frey, Hans Mattias Larsson, François Gorostidi

Although urothelial progenitor-like cells have been described in the human urinary tract, the existence of stem cells remains to be proven. Using a culture system that favors clonogenic epithelial cell growth, we evaluated and characterized clonal human ur ...

Public Library of Science2014

Tubular collagen-based and collagen-PCL scaffolds with seeded smooth muscle cell for urethra repair in rabbits

Peter Frey

Wiley-Blackwell2014

Engineered fibrin based scaffolds for urological tissue engineering applications

Jeffrey Alan Hubbell, Peter Frey, Elif Vardar

Wiley-Blackwell2014

Human and porcine urothelial stem cells

Jeffrey Alan Hubbell, Yann Barrandon, Peter Frey, Hans Mattias Larsson, François Gorostidi

Wiley-Blackwell2014

Engineered insulin-like growth factor-1 for improved smooth muscle regeneration

Lirong Yang, Jeffrey Alan Hubbell, Peter Frey, Kristen Lorentz

Insulin-like growth factor-1 (IGF-1) has been shown to induce potent mitogenic responses in various cell types, yet its sustained local delivery is still an underdeveloped domain in the clinic. We report here an engineered IGF-1 that facilitates extended l ...

2012