Publications de Virginie Bachmann

Bacterial matrix metalloproteases and serine proteases contribute to the extra-host inactivation of enteroviruses in lake water

Enteroviruses are ubiquitous contaminants of surface waters, yet their fate in presence of microbial congeners is poorly understood. In this work, we investigated the inactivation of Echovirus-11 (E11) and Coxsackievirus-A9 (CVA9) by bacteria isolated from ...

2022

Adaptation of Human Enterovirus to Warm Environments Leads to Resistance against Chlorine Disinfection

Virginie Bachmann, Tamar Kohn, Anna Carratala Ripolles, Timothy R. Julian

Sunlight, temperature, and microbial grazing are among the environmental factors promoting the inactivation of viral pathogens in surface waters. Globally, these factors vary across time and space. The persistence of viral pathogens, and ultimately their e ...

2020

Viral Transfer and Inactivation through Zooplankton Trophic Interactions

Virginie Bachmann, Tamar Kohn, Margot Ninon Lauren Olive, Xavier Fernandez Cassi

Waterborne viruses are responsible for numerous diseases and are abundant in aquatic systems. Understanding the fate of viruses in natural systems has important implications for human health. This research quantifies the uptake of bacteriophage T4 and the ...

2020

Relationship Between Inactivation and Genome Damage of Human Enteroviruses Upon Treatment by UV254, Free Chlorine, and Ozone

Virginie Bachmann, Tamar Kohn, Jason Robert Torrey, Suzanne Marie Young

Quantitative PCR (qPCR) is a convenient tool for monitoring virus concentrations in water and wastewater treatment trains, though it only informs about virus presence, but not infectivity. This limitation can be overcome if the relationship between infecti ...

2019

Experimental adaptation of human echovirus 11 to ultraviolet radiation leads to tolerance to disinfection and resistance to ribavirin

Virginie Bachmann, Tamar Kohn, Hyunjin Shim, Qingxia Zhong, Jeffrey David Jensen, Anna Carratala Ripolles

Ultraviolet light in the UVC range is a commonly used disinfectant to control viruses in clinical settings and water treatment. Here, we employed an experimental evolution approach to investigate the adaptation of human echovirus 11 to disinfection by UVC, ...

2017

Resistance of echovirus 11 to ClO2 is associated with enhanced host receptor use, altered entry routes and high fitness

Virginie Bachmann, Tamar Kohn, Hyunjin Shim, Qingxia Zhong, Jeffrey David Jensen, Anna Carratala Ripolles

Waterborne viruses can exhibit resistance to common water disinfectants, yet the mechanisms that allow them to tolerate disinfection are poorly understood. Here, we generated echovirus 11 (E11) with resistance to chlorine dioxide (ClO2) by experimental evo ...

Amer Chemical Soc2017

Cross-resistance of UV- or chlorine dioxide-resistant echovirus 11 to other disinfectants

Virginie Bachmann, Tamar Kohn, Qingxia Zhong, Anna Carratala Ripolles, Rachele Ossola

The emergence of waterborne viruses with resistance to disinfection has been demonstrated in the laboratory and in the environment. Yet, the implications of such resistance for virus control remain obscure. In this study we investigate if viruses with resi ...

Frontiers Media SA2017

Genetic, structural and phenotypic properties of MS2 coliphage with resistance to ClO2 disinfection

Virginie Bachmann, Tamar Kohn, Qingxia Zhong, Petr Leiman, Sergey Nazarov, Anna Carratala Ripolles, Laura Piccinini, Ricardo Cesar Guerrero Ferreira

Common water disinfectants like chlorine have been reported to select for resistant viruses, yet little attention has been devoted to characterizing disinfection resistance. Here, we investigated the resistance of MS2 coliphage to inactivation by chlorine ...

Amer Chemical Soc2016

A simple plasmid-based transient gene expression method using High Five cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Virginie Bachmann, Xiao Shen

The High Five (H5) cell line, derived from the lepidopteran Trichoplusia ni, is one of the major insect cell hosts for the production of recombinant proteins using the baculovirus expression vector system (BEVS). Here, we describe a simple polyethylenimine ...

Elsevier2015

CHO cell lines generated by PiggyBac transposition

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Virginie Bachmann

2011

Rapid recombinant protein production from pools of transposon-generated CHO cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Virginie Bachmann

2011