Publications de Etienne Shaffer

Digital holographic microscopy: a quantitative label-free microscopy technique for phenotypic screening

Gerardo Turcatti, Benjamin Rappaz, Etienne Shaffer

Digital Holographic Microscopy (DHM) is a label-free imaging technique allowing visualization of transparent cells with classical imaging cell culture plates. The quantitative DHM phase contrast image provided is related both to the intracellular refractiv ...

2014

Label-Free Cytotoxicity Screening Assay by Digital Holographic Microscopy

Gerardo Turcatti, Christian Depeursinge, Marc Chambon, Jonas Kühn, Pierre Magistretti, Benjamin Rappaz, Julien Mena, Jérôme Parent, Etienne Shaffer

We introduce a label-free technology based on digital holographic microscopy (DHM) with applicability for screening by imaging, and we demonstrate its capability for cytotoxicity assessment using mammalian living cells. For this first high content screenin ...

Mary Ann Liebert2013

Single-shot, simultaneous incoherent and holographic microscopy

Christian Depeursinge, Nicolas Pavillon, Etienne Shaffer

In this work, we demonstrate single-shot, simultaneous recording and subsequent retrieval of one incoherent and two holographic (intensity and phase) images from the same camera frame. Demultiplexing of incoherent and holographic signals in the spatial fre ...

Wiley-Blackwell2012

Holographic Second Harmonic Generation Microscopy

Etienne Shaffer

This dissertation is dedicated to the application of digital holography methods to second harmonic generation microscopy, and to the development of the resulting imaging technique: holographic second harmonic generation microscopy. Second harmonic generati ...

EPFL2011

Sub-Rayleigh resolution by phase imaging

Christian Depeursinge, Nicolas Pavillon, Etienne Shaffer, Yann Jérôme Michel Pascal Cotte, Muhammed Fatih Toy

We report the experimental observation of systematically occurring phase singularities in coherent imaging of sub-Rayleigh distanced objects. A theory that relates the observation to the sub-Rayleigh distance is presented and compared with experimental mea ...

Optical Society of America2010

Endoscopic low coherence topography measurement for upper airways and hollow samples

Christian Depeursinge, Jonas Kühn, Yves Delacrétaz, Nicolas Pavillon, Etienne Shaffer, Florian Lang

To evaluate the severity of airway pathologies, quantitative dimensioning of airways is of utmost importance. Endoscopic vision gives a projective image and thus no true scaling information can be directly deduced from it. In this article, an approach base ...

SPIE Society of Photo-optical Instrumentation Engineers2010

Real time, nanometric 3D-tracking of nanoparticles made possible by second harmonic generation digital holographic microscopy

Christian Depeursinge, Pierre Marquet, Etienne Shaffer

In the past decade, quantitative phase imaging gave a new dimension to optical microscopy, and the recent extension of digital holography techniques to nonlinear microscopy appears very promising, for the phase of nonlinear signal provides additional infor ...

Optical Society of America2010

Method and apparatus for retrieval of amplitude and phase of nonlinear electromagnetic waves

Christian Depeursinge, Etienne Shaffer

The present invention discloses a method and its associated apparatus to retrieve the amplitude and, especially, the phase of nonlinear electromagnetic waves. The application field of the present invention is optical imaging. A sample is probed by coherent ...

2010

Label-free second harmonic phase imaging of biological specimen by digital holographic microscopy

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Corinne Moratal, Etienne Shaffer

We have previously developed a new way for nonscanning second-harmonic generation (SHG) microscopy [Opt. Lett. 34, 2450 (2009)]. Based on digital holography, this technique captures, in single-shot hologram acquisition, both the amplitude and the phase of ...

Optical Society of America2010

Digital holographic microscopy investigation of second harmonic generated at a glass/air interface

Christian Depeursinge, Jonas Kühn, Nicolas Pavillon, Etienne Shaffer

Optical second harmonic generation, thanks to its coherent nature, is a suitable signal for interferometric measurements, such as digital holography: a well-established imaging technique that allows recovering complex diffraction wavefields from which it i ...

2009