Publications de Howard Riezman

Type 2 diabetes disrupts circadian orchestration of lipid metabolism and membrane fluidity in human pancreatic islets

Recent evidence suggests that circadian clocks ensure temporal orchestration of lipid homeostasis and play a role in pathophysiology of metabolic diseases in humans, including type 2 diabetes (T2D). Nevertheless, circadian regulation of lipid metabolism in ...

PUBLIC LIBRARY SCIENCE2022

Mitochondria-specific photoactivation to monitor local sphingosine metabolism and function

Howard Riezman

Photoactivation ('uncaging') is a powerful approach for releasing bioactive small molecules in living cells. Current uncaging methods are limited by the random distribution of caged molecules within cells. We have developed a mitochondria-specific photoact ...

2018

A Chemogenetic Approach for the Optical Monitoring of Voltage in Neurons

Rudolf Hovius, Kai Johnsson, Luc Reymond, Howard Riezman, Efthymia Prifti

Wiley-VCH Verlag GmbH2018

Lipidomics reveals diurnal lipid oscillations in human skeletal muscle persisting in cellular myotubes cultured in vitro

Marc Moniatte, Howard Riezman, Jonathan Paz Montoya, Frédéric Bruno Martin Gachon, Benjamin Dieter Weger

Circadian clocks play an important role in lipid homeostasis, with impact on various metabolic diseases. Due to the central role of skeletal muscle in whole-body metabolism, we aimed at studying muscle lipid profiles in a temporal manner. Moreover, it has ...

National Academy of Sciences2017

A method for analysis and design of metabolism using metabolomics data and kinetic models: Application on lipidomics using a novel kinetic model of sphingolipid metabolism

Paolo Angelino, Vassily Hatzimanikatis, Howard Riezman, Georgios Savoglidis

We present a model-based method, designated Inverse Metabolic Control Analysis (IMCA), which can be used in conjunction with classical Metabolic Control Analysis for the analysis and design of cellular metabolism. We demonstrate the capabilities of the met ...

Academic Press Inc Elsevier Science2016

Systematic lipidomic analysis of yeast protein kinase and phosphatase mutants reveals novel insights into regulation of lipid homeostasis

Howard Riezman, Fabrice David, Manuele Piccolis

The regulatory pathways required to maintain eukaryotic lipid homeostasis are largely unknown. We developed a systematic approach to uncover new players in the regulation of lipid homeostasis. Through an unbiased mass spectrometry-based lipidomic screening ...

Amer Soc Cell Biology2014

A fluorogenic probe for SNAP-tagged plasma membrane proteins based on the solvatochromic molecule Nile Red

Rudolf Hovius, Kai Johnsson, Luc Reymond, Howard Riezman, Efthymia Prifti

A fluorogenic probe for plasma membrane proteins based on the dye Nile Red and SNAP-tag is introduced. It takes advantage of Nile Red, a solvatochromic molecule highly fluorescent in an apolar environment, such as cellular membranes, but almost dark in a p ...

Amer Chemical Soc2014

Activation of the unfolded protein response pathway causes ceramide accumulation in yeast and INS-1E insulinoma cells

Howard Riezman

Sphingolipids are not only important components of membranes but also have functions in protein trafficking and intracellular signaling. The LCB1 gene encodes a subunit of the serine palmitoyltransferase, which is responsible for the first step of sphingol ...

2012

Glycosylphosphatidylinositol anchors regulate glycosphingolipid levels

Howard Riezman

Glycosylphosphatidylinositol (GPI) anchor biosynthesis takes place in the endoplasmic reticulum (ER). After protein attachment, the GPI anchor is transported to the Golgi where it undergoes fatty acid remodeling. The ER exit of GPI-anchored proteins is con ...

2012

Chemical Biology Approaches to Membrane Homeostasis and Function

Gerardo Turcatti, Kai Johnsson, Luc Reymond, Christian Heinis, Stefan Matile, Howard Riezman

The study of membranes is at a turning point. New theories about membrane structure and function have recently been proposed, however, new technologies, combining chemical, physical, and biochemical approaches are necessary to test these hypotheses. In par ...

2011

NCCR Chemical Biology: Interdisciplinary Research Excellence, Outreach, Education, and New Tools for Switzerland

Kai Johnsson, Howard Riezman

Funded by the Swiss National Science Foundation to promote cutting edge research as well as the advancement of young researchers and women, technology transfer, outreach and education, the NCCR (Swiss National Centre of Competence in Research) Chemical Bio ...

2011