Publications de Liang Qiao | EPFL Graph Search

Detection of antimicrobial resistance-associated proteins by titanium dioxide-facilitated intact bacteria mass spectrometry

Hubert Girault, Horst Pick, Liang Qiao, Natalia Gasilova, Yingdi Zhu, Milica Jovic

Titanium dioxide-modified target plates were developed to enhance intact bacteria analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The plates were designed to photocatalytically destroy the bacterial envelope struct ...

2018

On-Chip Spyhole Nanoelectrospray Ionization Mass Spectrometry for Sensitive Biomarker Detection in Small Volumes

Hubert Girault, Liang Qiao, Géraldine Margaretha Alice Stauffer, Xiaoqin Zhong

A polyimide microfluidic chip with a microhole emitter (Ø 10–12 μm) created on top of a microchannel by scanning laser ablation has been designed for nanoelectrospray ionization (spyhole-nanoESI) to couple microfluidics with mass spectrometry. The spyhole- ...

2018

Maldi device and methods of preparation and use thereof

Hubert Girault, Horst Pick, Liang Qiao, Yingdi Zhu, Milica Jovic

A target plate for enhanced matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) analysis of intact biological entities. The target plate comprises an electrically conductive substrate (1) covered at least partially with immobilised pho ...

2018

Bacterial Whole Cell Typing by Mass Spectra Pattern Matching with Bootstrapping Assessment

Hubert Girault, Yu Lin, Liang Qiao

Bacterial typing is of great importance in clinical diagnosis, environmental monitoring, food safety analysis, and biological research. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is now widely used to analyze bacterial samples ...

American Chemical Society2017

Electrostatic spray ionization from 384-well microtiter plates for mass spectrometry analysis based enzyme assay and drug metabolism screening

Hubert Girault, Liang Qiao, Elena Tobolkina, Tzu-En Lin, Xiaoqin Zhong, Yan Deng, Emna Belghith

We have realized the direct ionization of samples from wells of microtiter plates under atmospheric conditions for mass spectrometry analysis without any liquid delivery system or any additional interface. The microtiter plate is a commercially available 3 ...

American Chemical Society2017

Sensitive and fast identification of bacteria in blood samples by immunoaffinity mass spectrometry for quick BSI diagnosis

Hubert Girault, Horst Pick, Michel Prudent, Niels Erik Lion, Liang Qiao, Natalia Gasilova, Alexandra Bondarenko, Yingdi Zhu

Bloodstream infections rank among the most serious causes of morbidity and mortality in hospitalized patients, partly due to the long period (up to one week) required for clinical diagnosis. In this work, we have developed a sensitive method to quickly and ...

Royal Soc Chemistry2016

On-chip mesoporous functionalized magnetic microspheres for protein sequencing by extended bottom-up mass spectrometry

Hubert Girault, Yury Tsybin, Liang Qiao, Natalia Gasilova, Kristina Srzentic

Limited amount and extreme concentration variability of proteomic-related samples require efficient analyte preconcentration and purification prior to the mass spectrometry (MS)-based analysis. Preferably, these steps should be coupled on-line with chosen ...

American Chemical Society2016

Open channel-based microchip electrophoresis interfaced with mass spectrometry via electrostatic spray ionization

Hubert Girault, Liang Qiao, Natalia Gasilova, Yan Deng

The coupling between open channel-based microchip electrophoresis and mass spectrometry via electrostatic spray ionization is proposed for in situ detection of fractionated analytes. Electrophoretic separation is performed in an open channel fabricated in ...

Elsevier2016

Mass Barcode Signal Amplification for Multiplex Allergy Diagnosis by MALDI-MS

Hubert Girault, Liang Qiao, Natalia Gasilova, Xiaoqin Zhong

A highly sensitive method based on mass-barcoded gold nanoparticles (AuNPs) and immunomagnetic separation has been developed for multiplex allergy diagnosis by MALDI mass spectrometry in a component-resolved manner. Different analytical probes were prepare ...

American Chemical Society2016

Electrostatic Spray Ionization Method

Romain Sartor, Yu Lu, Liang Qiao, Elena Tobolkina

In an electrostatic spray ionization method for spraying a liquid layer from an insulating plate 2, the plate is arranged between two electrodes 1, 4. A constant high voltage power supply 3 is provided and an electric circuit is used to charge and discharg ...

2015

Multiple scanning electrochemical microscopy mapping of tyrosinase in micro-contact printed fruit samples on polyvinylidene fluoride membrane

Hubert Girault, Fernando Cortes Salazar, Liang Qiao, Andreas Stephan Lesch, Alexandra Bondarenko, Tzu-En Lin

Herein, we introduce three orthogonal and compatible methods for detecting tyrosinase, a key factor in fruit browning and skin disorders, with high spatial resolution by means of scanning electrochemical microscopy (SECM). All methods are performed subsequ ...

Elsevier2015

Ambient in situ analysis and imaging of both hydrophilic and hydrophobic thin layer chromatography plates by electrostatic spray ionization mass spectrometry

Hubert Girault, Liang Qiao, Xiaoqin Zhong

Electrostatic spray ionization (ESTASI) mass spectrometry imaging (MSI), recently developed to profile spatial distribution of chemical and biochemical molecules on an insulating surface under ambient conditions, has been applied to identify and quantify c ...

RSC Publishing2015

Porous silica enhanced proteolysis during Off-Gel separation for efficient protein identification

Hubert Girault, Mohammad Reza Pourhaghighi, Liang Qiao

An advanced approach is developed in this work for simultaneous on-line separation and digestion of proteins by combining the Off-Gel isoelectric focusing (IEF) and enzymatic nanoreactor enhanced proteolysis. The nanoreactor was prepared by preloading tryp ...

Elsevier2015

Standard addition strip for quantitative electrostatic spray ionization mass spectrometry analysis: Determination of caffeine in drinks

Hubert Girault, Christophe Roussel, Liang Qiao, Elena Tobolkina

Standard addition strips were prepared for the quantitative determination of caffeine in different beverages by electrostatic spray ionisation mass spectrometry (ESTASI-MS). The gist of this approach is to dry spots of caffeine solutions with different con ...

Elsevier2014

Surprising acidity of hydrated lithium cations in organic solvents

Hubert Girault, Liang Qiao, Haiqiang Deng, Thomas James Stockmann, Pekka Eero Peljo

Lithium cations are shown to have a significant role on catalyzing oxygen and proton reduction along with SN1 reactions in biphasic systems. We propose that this catalytic effect is due to the surprising acidity of the hydrated cations; interactions betwee ...

Royal Society of Chemistry2014

Fingerprinting the Tertiary Structure of Electroadsorbed Lysozyme at Soft Interfaces by Electrostatic Spray Ionization Mass Spectrometry

Hubert Girault, Liang Qiao, Micheal Diarmaid Scanlon

Lysozyme can be electrochemically detected after adsorption at an electrified gel-water interface. Ex-situ characterization by electrostatic spray ionization mass spectrometry provides insights into the interfacial detection mechanism by allowing changes t ...

Royal Society of Chemistry2014

On-Chip Spyhole Mass Spectrometry for Droplet-Based Microfluidics

Hubert Girault, Liang Qiao, Natalia Gasilova, Qiuliyang Yu

For efficient coupling of droplet-based microfluidics with mass spectrometry (MS), a spyhole drilled on the top of a microchip is used to sample the passing droplets by electrostatic-spray ionization (ESTASI) MS. The technique involves placing an electrode ...

Wiley-VCH Verlag GmbH2014

An Efficient Drug Metabolism Strategy Based on Microsome-Mesoporous Organosilica Nanoreactors

Hubert Girault, Liang Qiao, Peng Zhang

A rapid and accurate in-vitro drug metabolism strategy has been proposed based on the design of a biomimetic nanoreactor composed of amino-functionalized periodic mesoporous organosilica (NH2-PMO) and microsomes. The amphiphilic and positive charged NH2-PM ...

Amer Chemical Soc2014

Highly sensitive detection of five typical fluoroquinolones in low-fat milk by field-enhanced sample injection based CE in bubble cell capillary

Hubert Girault, Liang Qiao, Natalia Gasilova, Yan Deng

Fluoroquinolones are a group of synthetic antibiotics with a broad activity spectrum against mycoplasma, gram-positive and gram-negative bacteria. Due to the extensive use of fluoroquinolones in farming and veterinary science, there is a constant need in t ...

Wiley-Blackwell2014

Electrostatic Spray Ionization Mass Spectrometry Imaging

Hubert Girault, Horst Vogel, Horst Pick, Liang Qiao, Elena Tobolkina, Andreas Stephan Lesch, Alexandra Bondarenko, Xiaoqin Zhong

Imaging samples on a surface by mass spectrometry (MS) requires the combination of MS detection with a scanning mode that enables localized desorption and ionization and/or detection of sample analytes with good spatial resolution. We have developed a new ...

Amer Chemical Soc2014