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Liang Qiao

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Publications associées (47)

Proteins/peptides purification by a three-well OFFGEL electrophoresis with immobilized ultra narrow pH gradient gels

Hubert Girault, Fernando Cortes Salazar, Liang Qiao, Elena Tobolkina

Purification and desalting of protein and peptide samples by a three-well OFFGEL electrophoresis with immobilized ultra narrow pH gradient gels is proposed as a fast preparative strategy for proteomics. The gist of this strategy is to separate the proteins ...

Royal Society of Chemistry2014

A microchip emitter for solid phase extraction – gradient elution – mass spectrometry

Hubert Girault, Mohammad Reza Pourhaghighi, Liang Qiao, Dmitry Momotenko, Natalia Gasilova

A microchip electrospray emitter with a magnetic bead trap has been designed for solid phase extraction-gradient elution-mass spectrometry (SPE-GEMS). The goal of this method is the detection of analytes at low concentrations and it is here demonstrated us ...

American Chemical Society2013

Coupling Isoelectric Focusing Gel Electrophoresis to Mass Spectrometry by Electrostatic Spray Ionisation

Hubert Girault, Liang Qiao, Elena Tobolkina

Gel electrophoresis has been used for decades as a high-resolution separation technique for proteins and protein isomers, but limited in the coupling with MS because of low throughput and poor automaticity compared with LC-MS. In this work, we have develop ...

American Chemical Society2013

Amino-functionalized macroporous silica for efficient tryptic digestion in acidic solutions

Hubert Girault, Jingjing Wan, Liang Qiao

Amino-functionalized macroporous silica foam (NH2-MOSF) has been developed as a host reactor to realize highly efficient proteolysis in acidic solutions where normal tryptic reactions cannot occur. The digestion protocol consists simply of adding the funct ...

Wiley-Blackwell2013

Electrostatic-spray ionization mass spectrometry sniffing for perfume fingerprinting

Hubert Girault, Liang Qiao, Elena Tobolkina

RATIONALE: The perfume market is growing significantly, and it is easy to find imitative fragrances of probably all types of perfume. Such imitative fragrances are usually of lower quality than the authentic ones, creating a possible threat for perfume com ...

Wiley-Blackwell2013

Electrochemically Controlled Proton-Transfer-Catalyzed Reactions at Liquid-Liquid Interfaces: Nucleophilic Substitution on Ferrocene Methanol

Hubert Girault, Liang Qiao, Pekka Eero Peljo

The generation of α-ferrocenyl carbocations from ferrocenyl alcohols for SN1 substitution at the water–organic solvent interface is initiated by the transfer of protons into the organic phase. The proton flux, and hence the reaction rate, can be controlled ...

Wiley-Blackwell2013

Mass Spectrometry based Analytical Methods for Proteome Analysis and Redoxomics

Liang Qiao

Proteomics is nowadays one of the most important research topics in life science for understanding many life activities from the molecular level. The objective is to find out proteins or modifications on proteins that regulate biological events, aging, dis ...

EPFL2013

Antioxidant Promotion of Tyrosine Nitration in the Presence of Copper(II)

Hubert Girault, Liang Qiao

Copper(II) is known to catalyze the generation of reactive nitrogen species in the presence of hydrogen peroxide, nitrite or nitric oxide, leading to tyrosine nitration, a biomarker for free radical species associated diseases. Here, we find that biologica ...

Royal Society of Chemistry2013

Compatible Buffer for Capillary Electrophoresis and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Hubert Girault, Hong-Xu Chen, Liang Qiao, Natalia Gasilova

A compatible buffer system for the coupling of capillary electrophoresis (CE) with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was developed. The employed interface consists of a robot to drive a sliver-covered separation capil ...

Royal Society of Chemistry2013

Electrostatic-Spray Ionization Mass Spectrometry

Hubert Girault, Romain Sartor, Yu Lu, Liang Qiao, Elena Tobolkina, Natalia Gasilova

An electrostatic-spray ionization (ESTASI) method has been used for mass spectrometry (MS) analysis of samples deposited in or on an insulating substrate. The ionization is induced by a capacitive coupling between an electrode and the sample. In practice, ...

Amer Chemical Soc2012

Improved Conversion Rates in Drug Screening Applications Using Miniaturized Electrochemical Cells with Frit Channels

Hubert Girault, Albert van den Berg, Liang Qiao

This paper reports a novel design of a miniaturized three-electrode electrochemical cell, the purpose of which is aimed at generating drug metabolites with a high conversion efficiency. The working electrode and the counter electrode are placed in two sepa ...

Amer Chemical Soc2012

Electrochemical Push–Pull Scanner with Mass Spectrometry Detection

Hubert Girault, Gunther Wittstock, Fernando Cortes Salazar, Liang Qiao, Dmitry Momotenko, Andreas Stephan Lesch

This manuscript presents a push-pull electrochem. scanner able to image reactivity of initially dry surfaces by scanning electrochem. microscopy (SECM) and to probe mols. present or generated at the surface by mass spectrometry (MS). The proof-of-concept i ...

Amer Chemical Soc2012

Proteolysis in microfluidic droplets: an approach to interface protein separation and peptide mass spectrometry

Hubert Girault, Liang Qiao

A versatile microreactor protocol based on microfluidic droplets has been developed for on-line protein digestion. Proteins separated by liquid chromatography are fractionated in water-in-oil droplets and digested in sequence. The microfluidic reactor acts ...

Royal Society of Chemistry2012

Copper-Catalyzed Tyrosine Nitration

Hubert Girault, Yu Lu, Liang Qiao

Tyrosine nitration, often observed during neurodegenerative disorders under nitrative stress, is usually considered to be induced chemically either by nitric oxide and oxygen forming nitrogen dioxide or by the decomposition of peroxynitrite. It can also be ...

2011

LEPA: From Proteomics to Energy Conversion

Hubert Girault, Anne-Laure Gassner, Fernando Cortes Salazar, Mohammad Reza Pourhaghighi, Manuel Alejandro Méndez Agudelo, Liang Qiao

This review article summarizes the different fields of research at the Laboratoire d'Electrochimie Physique et Analytique at the Ecole Polytechnique Federale de Lausanne. The research areas covered include charge transfer reactions at soft interfaces, bio- ...

2011

Controlling the specific enrichment of multi-phosphorylated peptides on oxide materials: aluminium foil as a target plate for laser desorption ionization mass spectrometry

Hubert Girault, Mohamad Hojeij, Hongyan Bi, Manuel Alejandro Méndez Agudelo, Liang Qiao

A specific multi-phosphopeptide enrichment method is presented to complement current phosphopeptide isolation strategies that normally bias mass spectrometry analysis towards the detection of mono-phosphopeptides. An adsorption model is developed that show ...

2010

Electrochemical reactions and ionization processes

Hubert Girault, Michel Prudent, Niels Erik Lion, Mélanie Abonnenc, Hongyan Bi, Liang Qiao

Electrochemical or photo-electrochemical reactions in both electrospray ionization and laser desorption ionization are discussed stressing the role of the electrode reaction in influencing the ionization process. In particular, upon application of a high v ...

2010

Copper(I) and copper(II) binding to β-amyloid 16 (Aβ16) studied by electrospray ionization mass spectrometry

Hubert Girault, Michel Prudent, Yu Lu, Liang Qiao

Copper-b-amyloid 16 (Aβ16) complexes were investigated by electrospray ionization mass spectrometry (ESI-MS). Copper(I) and (II) complexes were formed on-line in a microchip electrospray emitter by using a sacrificial copper electrode as the anode in posit ...

Royal Society of Chemistry2010

Electrochemical Aspects of Electrospray and Laser Desorption/Ionization for Mass Spectrometry

Hubert Girault, Mélanie Abonnenc, Liang Qiao

Soft-ionization methods, namely electrospray ionization and laser desorption/ionization, are widely used to transfer large molecules as intact gas-phase ions either from a solution or from a solid substrate. During both processes, in-source electrochemical ...

2010

Nanomaterial-assisted laser desorption ionization for mass spectrometry-based biomedical analysis

Hubert Girault, Liang Qiao

Nanomaterials have been widely used to assist laser desorption ionization of biomolecules for mass spectrometry analysis. Compared with classical matrix-assisted laser desorption ionization, strategies based on nanomaterial-assisted ionization generate a c ...

2010

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