Publications de Cristina Diaz Perlas

Structure‐affinity relationship analysis and affinity maturation of a calprotectin‐binding peptide

Christian Heinis, Lluc Farrera Soler, Cristina Diaz Perlas

Peptide 3 is an 18‐amino acid linear peptide binding with sub‐micromolar affinity to the inflammation marker calprotectin. Its application in point‐of‐care diagnostic assays has shown promising results, yet improving its affinity for calprotectin could fac ...

Wiley2025

High-affinity peptides developed against calprotectin and their application as synthetic ligands in diagnostic assays

Christian Heinis, Florence Pojer, Lluc Farrera Soler, Kelvin Ka Ching Lau, Cristina Diaz Perlas

A peptide was developed that binds to calprotectin, a marker of major inflammatory disorders, and found to be suited for diagnostic tests. The use of synthetic peptides in assays is of great interest due to their high precision, robustness and low price. ...

Nature Portfolio2023

Synthesis and direct assay of large macrocycle diversities by combinatorial late-stage modification at picomole scale

Gerardo Turcatti, Jonathan Patrice Vesin, Christian Heinis, Alessandro Angelini, Gontran Sangouard, Julien Bortoli Chapalay, Mischa Schüttel, Ganesh Kumar Mothukuri, Manuel Leonardo Merz, Sevan Mleh Habeshian, Cristina Diaz Perlas, Zsolt Bognár

Macrocycles have excellent potential as therapeutics due to their ability to bind challenging targets. However, generating macrocycles against new targets is hindered by a lack of large macrocycle libraries for high-throughput screening. To overcome this, ...

Nature Research2022

Phage display selection of peptides against the inflammation marker calprotectin and their application in a diagnostic assay

Christian Heinis, Cristina Diaz Perlas

WILEY2022

Picomole-Scale Synthesis and Screening of Macrocyclic Compound Libraries by Acoustic Liquid Transfer

Gerardo Turcatti, Jonathan Patrice Vesin, Christian Heinis, Alessandro Zorzi, Gontran Sangouard, Julien Bortoli Chapalay, Sangram Shivdas Kale, Mischa Schüttel, Yu Teng Wu, Cristina Diaz Perlas

Macrocyclic compounds are an attractive class of therapeutic ligands against challenging targets, such as protein-protein interactions. However, the development of macrocycles as drugs is hindered by the lack of large combinatorial macrocyclic libraries, w ...

Wiley-VCH Verlag GmbH2021

Generation of a 100-billion cyclic peptide phage display library having a high skeletal diversity

Christian Heinis, Xudong Kong, Vanessa Carle, Alice Comberlato, Cristina Diaz Perlas

Phage display is a powerful technique routinely used for the generation of peptide- or protein-based ligands. The success of phage display selections critically depends on the size and structural diversity of the libraries, but the generation of large libr ...

2021

Development of Selective FXIa Inhibitors Based on Cyclic Peptides and Their Application for Safe Anticoagulation

Christian Heinis, Camille Villequey, Xudong Kong, Kaycie Marie Butler, Vanessa Carle, Christina Lamers, Tamara Maric, Chloé Rogg, Cristina Diaz Perlas

Coagulation factor XI (FXI) has emerged as a promising target for the development of safer anticoagulation drugs that limit the risk of severe and life-threatening bleeding. Herein, we report the first cyclic peptide-based FXI inhibitor that selectively an ...

2021

Generation of a Large Peptide Phage Display Library by Self-Ligation of Whole-Plasmid PCR Product

Christian Heinis, Xudong Kong, Kaycie Marie Butler, Vanessa Carle, Cristina Diaz Perlas

The success of phage display, used for developing target-specific binders based on peptides and proteins, depends on the size and diversity of the library screened, but generating large libraries of phage-encoded polypeptides remains challenging. New pepti ...

2020