Publications associées à Optofluidics of plants

Electronic readout of DNA amplification in nanoliter chambers. Strategies towards highly parallel semiconductor-based nucleic acid amplification testing

Polymerase chain reaction (PCR) has been the most significant driver in the field of nucleic acid testing (NAT) since its invention. Popularized as an abbreviation by the Covid-19 pandemic, PCR-based methods are the gold standard in the field of diagnostic ...

EPFL2023

Microfluidic-based linear-optics label-free imager

Demetri Psaltis

Linear optics based nanoscopy previously reached resolution beyond the diffraction limit, illuminating samples in the visible light regime while allowing light to interact with freely moving metallic nanoparticles. However, the hydrodynamics governing the ...

ROYAL SOC CHEMISTRY2020

Plant responses to rising vapor pressure deficit

Charlotte Grossiord

Recent decades have been characterized by increasing temperatures worldwide, resulting in an exponential climb in vapor pressure deficit (VPD). VPD has been identified as an increasingly important driver of plant functioning in terrestrial biomes including ...

2020

Contactless optical trapping and manipulation of nanoparticles utilizing SIBA mechanism and EDL force

Mahdi Sahafi

On-chip optical tweezers based on evanescent fields overcome the diffraction limit of the free-space optical tweezers and can be a promising technique for developing lab-on-a-chip devices. While such trapping allows for low-cost and precise manipulation, i ...

2019

Lab-on-a-chip technologies for manipulation and imaging of C. elegans worms and embryos

Li Dong

The nematode Caenorhabditis elegans is an attractive model organism, owing notably to its short life cycle, genetic tractability, and optical transparency facilitating microscopic observation. This thesis deals with the realization of technological tools f ...

EPFL2018

Microfluidic and Electrokinetic Manipulation of Single Cells

Samuel Kilchenmann

Traditional cell assays report on the average results of a cell population. However, a wide range of new tools are being developed for a fundamental understanding of single cell's functionality. Nonetheless, the current tools are either limited in their th ...

EPFL2017

Microfluidic systems for high-throughput and high-content screening using the nematode Caenorhabditis elegans

Thomas Lehnert, Martinus Gijs, Matteo Cornaglia

In a typical high-throughput drug screening (HTS) process, up to millions of chemical compounds are applied to cells cultured in well plates, aiming to find molecules that exhibit a robust dose-response, as evidenced for example by a fluorescence signal. I ...

Royal Soc Chemistry2017

System for optical detection and imaging of sub-diffraction-limited nano-objects

Martinus Gijs, Hui Yang, Matteo Cornaglia

Nano-objects (8) with feature size smaller than the classical Abbe's diffraction limit can be optically detected via a microlens (4) array configured in a miniaturized microfluidic device. A hydrophilic microwell (3) array in a hydrophobic layer (2) is fir ...

2016

Piégeage optique résonant et analyse du mouvement de nanoparticules piégées dans des cristaux photoniques à cavités creuses

Mario Lucien Henri Tonin

Optical trapping was used for decades in biotechnology for a broad range of applications. It can be used for remote manipulation, application of pico-Newton forces and even study DNA processes. Nevertheless, optical trapping setups can be cumbersome. In or ...

EPFL2016

Merging microfluidics and micro-array concepts: from molecular to nematode-based bioassays

Matteo Cornaglia

Essential in biomedical research is the necessity of gathering statistically relevant data about large populations of specific biological entities, e.g. organisms, cells or molecules, while preserving detailed information about each single entity under in ...

EPFL2015

Microfluidic engineering of artificial stem cell niches

Simone Allazetta

Stem cells play a key role in a wide range of biological processes, in large part due to their ability to self-renew or differentiate into specialized cell types in response to various biological cues. In vivo, stem cells reside in a complex microenvironme ...

EPFL2015

A microfluidic flow-cell for the study of the ultrafast dynamics of biological systems

Majed Chergui, Adrien Alexis Paul Chauvet

The study of biochemical dynamics by ultrafast spectroscopic methods is often restricted by the limited amount of liquid sample available, while the high repetition rate of light sources can induce photodamage. In order to overcome these limitations, we de ...

American Institute of Physics2014

Lensfree optofluidic plasmonic sensor for real-time and label-free monitoring of molecular binding events over a wide field-of-view

Hatice Altug, Arif Engin Cetin

We demonstrate a high-throughput biosensing device that utilizes microfluidics based plasmonic microarrays incorporated with dual-color on-chip imaging toward real-time and label-free monitoring of biomolecular interactions over a wide field-of-view of > 2 ...

Nature Publishing Group2014

Crossed fiber optic Bessel beams for curvilinear optofluidic transport of dielectric particles

René Salathé, Fabrice Merenda, Kyunghwan Oh

Due to its unique non-diffracting and self-reconstructing nature, Bessel beams have been successfully adopted to trap multiple particles along the beam's axial direction. However, prior bulk-optic based Bessel beams have a fundamental form-factor limitatio ...

Optical Soc Amer2013

Angular Resolved low loss EELS for Materials Characterization

Simon Schneider

Electron Energy Loss Spectrometry (EELS) in Transmission Electron Microscopy (TEM) is a powerful tool for the investigation of the electronic structure of materials. In the low loss regime, one can access the optical properties which are governed by the di ...

EPFL2013

Application of Adaptive Optics to Focusing and Imaging

Daniel Iwaniuk

Adaptive optics is used to abate aberrations with a wavefront correction. It is widely used in astronomy and is starting to expand into applications of laser focusing and imaging with high numerical apertures, e.g. microscopy. The physical background of fo ...

EPFL2012

Optical Trapping in Microfluidic Channels for Functional and Chemical Analysis of Single Cells

Michael Werner

The physiological properties of cells are typically investigated in ensembles yielding averaged data that mask heterogeneities present in any cell population. Single-cell analysis techniques based on microfluidic "lab on a chip" (LOC) devices have recently ...

EPFL2011

Microfluidic array cytometer based on refractive optical tweezers for parallel trapping, imaging and sorting of individual cells

René Salathé, Horst Vogel, Fabrice Merenda, Joachim Piguet, Michael Werner

Analysis of genetic and functional variability in populations of living cells requires experimental techniques capable of monitoring cellular processes such as cell signaling of many single cells in parallel while offering the possibility to sort interesti ...

Royal Society of Chemistry2011

Controlling and utilizing optical forces at the nanoscale with plasmonic antennas

Olivier Martin, Andrea Lovera

Plasmonic dipole antennas are powerful optical devices for many applications since they combine a high field enhancement with outstanding tunability of their resonance frequency. The field enhancement, which is mainly localized inside the nanogap between b ...

Spie-Int Soc Optical Engineering, Po Box 10, Bellingham, Wa 98227-0010 Usa2011

Integration of plasmonic trapping in a microfluidic environment

Olivier Martin, Lina Huang, Sebastian Maerkl

Near field generated by plasmonic structures has recently been proposed to trap small objects. We report the first integration of plasmonic trapping with microfluidics for lab-on-a-chip applications. A three-layer plasmo-microfluidic chip is used to demons ...

Optical Society of America2009