Publications associées à Microfluidic Platforms for the Study of Bacterial Metabolism and Antimicrobial Susceptibility Testing

Homogeneous vs. heterogeneous photo-Fenton elimination of antibiotic-resistant bacteria bearing intracellular or extracellular resistance: Do resistance mechanisms interfere with disinfection pathways?

César Pulgarin, Stefanos Giannakis, Truong-Thien Melvin Le, Jérémie Decker

The present study aimed to fill the knowledge gap between the implications of intracellular and extracellular antibiotic resistance mechanisms may inflict on the inactivation pathways of the photo-Fenton process under mild conditions. It was thus designed ...

Elsevier Sci Ltd2024

Bactericidal effect of tetracycline in E. coli strain ED1a may be associated with ribosome dysfunction

Martin Beck, Camille Véronique Bernadette Goemans, Christian Eugen Zimmerli

Ribosomes translate the genetic code into proteins. Recent technical advances have facilitated in situ structural analyses of ribosome functional states inside eukaryotic cells and the minimal bacterium Mycoplasma. However, such analyses of Gram-negative b ...

Nature Research2024

Machine learning method for the classification of the state of living organisms' oscillations

Sandor Kasas, María Inés Villalba

The World Health Organization highlights the urgent need to address the global threat posed by antibiotic-resistant bacteria. Efficient and rapid detection of bacterial response to antibiotics and their virulence state is crucial for the effective treatmen ...

Frontiers Media Sa2024

Accurate and rapid antibiotic susceptibility testing using a machine learning-assisted nanomotion technology platform

Sandor Kasas, Eric Delarze, Florian Tagini, Gino Cathomen, Anthony Vocat, Laura Munch

Antimicrobial resistance (AMR) is a major public health threat, reducing treatment options for infected patients. AMR is promoted by a lack of access to rapid antibiotic susceptibility tests (ASTs). Accelerated ASTs can identify effective antibiotics for t ...

Nature Portfolio2024

Microfluidic systems for infectious disease diagnostics

Thomas Lehnert, Martinus Gijs

Microorganisms, encompassing both uni- and multicellular entities, exhibit remarkable diversity as omnipresent life forms in nature. They play a pivotal role by supplying essential components for sustaining biological processes across diverse ecosystems, i ...

Royal Soc Chemistry2024

Implementing water recirculation in a novel portable plasma-activated water reactor enhances antimicrobial effect against Escherichia coli

Alan Howling, Ivo Furno, Fabio Avino, Lorenzo Ibba, Rita Agus, Leonardo Zampieri, Brayden Graham Myers

The need for sustainable and reliable decontamination methods is driven by concerns regarding antibiotic resistance, as well as environmental and cost -efficiency challenges associated with traditional methods. Plasmaactivated water (PAW) holds significant ...

Elsevier Science Sa2024

Stability of influenza A virus in droplets and aerosols is heightened by the presence of commensal respiratory bacteria

Tamar Kohn, Htet Kyi Wynn, Céline Terrettaz, Aline Laetitia Schaub, Athanasios Nenes, Kalliopi Violaki, Marta Augugliaro, Shannon Christa David, Ghislain Gilles Jean-Michel Motos, Walter Hugentobler, Laura José Costa Henriques, Daniel Scott Nolan

Aerosol transmission remains a major challenge for control of respiratory viruses, particularly those causing recurrent epidemics, like influenza A virus (IAV). These viruses are rarely expelled alone, but instead are embedded in a consortium of microorgan ...

American Society for Microbiology2024

Multiscale Porosity Microfluidics to Study Bacterial Transport in Heterogeneous Chemical Landscapes

Francesco Carrara, Jiande Zhou

Microfluidic models are proving to be powerful systems to study fundamental processes in porous media, due to their ability to replicate topologically complex environments while allowing detailed, quantitative observations at the pore scale. Yet, while por ...

Wiley2024

Decomposition method to evaluate district heating/cooling network potential at urban scale

Luc Girardin, François Maréchal, Raphaël Briguet, Ana Catarina Gouveia Braz, Bingqian Liu

In this work, a tool to design district heating networks (DHN) is presented and applied to the city of Lausanne as a case study. The evaluation of the buildings’ heat/cooling demand is performed using a Geographic Information System (GIS) database, built f ...

Associazione Italiana Di Ingegneria Chimica2024

Design and assessment of an evaporating water-cooled methanation reactor for a SOE-based power-to-methane system

Philippe Aubin

The transition towards clean renewable energy sources, where wind and solar are prone to variation, requires adequate energy storage. Power-to-methane (PtM) systems can be part of the solution. Specifically, solid-oxide-electrolyser (SOE) based PtM systems ...

EPFL2024

Parity in bacterial communities and resistomes: Microplastic and natural organic particles in the Tyrrhenian Sea

Florian Frédéric Vincent Breider, Sylvain Coudret

Petroleum-based microplastic particles (MPs) are carriers of antimicrobial resistance genes (ARGs) in aquatic environments, influencing the selection and spread of antimicrobial resistance. This research characterized MP and natural organic particle (NOP) ...

2024

Improved microfluidic transcriptome based screening methods

Christoph Merten, Leonie Kolmar, Xiaoli Ma, Hongxing Hu

The invention provides microfluidic screening systems for the identification of compounds or compositions influencing cellular transcriptomes. The invention is predicated upon taking into account differences in newly synthesized mRNA in contrast to so call ...

2024

Simple optical nanomotion method for single-bacterium viability and antibiotic response testing

Sandor Kasas, María Inés Villalba, Allan Bonvallat, Eugenia Rossetti

Antibiotic resistance is nowadays a major public health issue. Rapid antimicrobial susceptibility tests (AST) are one of the options to fight this deadly threat. Performing AST with single-cell sensitivity that is rapid, cheap, and widely accessible, is ch ...

National Academy of Sciences2023

Antimicrobial susceptibility testing with integrated photonic crystal optical tweezers

Nicolas Villa

The abusive use of antimicrobial drugs during the last eighty years has favoured the natural selection of resistant pathogens able to neutralise drugs efficiently. Nowadays, antimicrobial resistance contributes to millions of deaths yearly, threatens the e ...

EPFL2023

Glacier-Fed Stream Biofilms Harbor Diverse Resistomes and Biosynthetic Gene Clusters

Leïla Ezzat, Vincent Henri De Staercke, Tom Ian Battin, Paraskevi Pramateftaki, Hannes Markus Peter, Matteo Tolosano, Michail Styllas, Tyler Joe Kohler, Stylianos Fodelianakis, Massimo Bourquin, Susheel Bhanu Busi, Grégoire Marie Octave Edouard Michoud

Antimicrobial resistance is an omnipresent phenomenon in the anthropogenically influenced ecosystems. However, its role in shaping microbial community dynamics in pristine environments is relatively unknown. ...

AMER SOC MICROBIOLOGY2023

DNA modifications impact natural transformation of Acinetobacter baumannii

Christian Iseli, Nicolas Jean Philippe Guex, Melanie Blokesch, Nina Vesel, Alexandre Lemopoulos

Acinetobacter baumannii is a dangerous nosocomial pathogen, especially due to its ability to rapidly acquire new genetic traits, including antibiotic resistance genes (ARG). In A. baumannii, natural competence for transformation, one of the primary modes o ...

2023

Single-Cell Optical Nanomotion of Candida albicans in Microwells for Rapid Antifungal Susceptibility Testing

Sandor Kasas, María Inés Villalba

Candida albicans is an emerging multidrug-resistant opportunistic pathogen representing an important source of invasive disease in humans and generating high healthcare costs worldwide. The development of a rapid and simple antifungal susceptibility test ( ...

MDPI2023

Material Substrate Physical Properties Control Pseudomonas aeruginosa Biofilm Architecture

Alice Cont, Alexandre Louis André Persat

The biofilm lifestyle is the most widespread survival strategy in the bacterial world. Pseudomonas aeruginosa biofilms cause chronic infections and are highly recalcitrant to antimicrobials. The genetic requirements allowing P. aeruginosa to grow into biof ...

AMER SOC MICROBIOLOGY2023

Biofilm formation on human immune cells is a multicellular predation strategy of Vibrio cholerae

Alexandre Louis André Persat, Sofya Mikhaleva

Biofilm formation is generally recognized as a bacterial defense mechanism against environmental threats, including antibiotics, bacteriophages, and leukocytes of the human immune system. Here, we show that for the human pathogen Vibrio cholerae, biofilm f ...

CELL PRESS2023

High-resolution imaging and analysis of the intestinal bacterial load of Caenorhabditis elegans during early adulthood

Martinus Gijs, Farzad Rezaeianaran

We study the presence within the worm Caenorhabditis elegans (C. elegans) of a fluorescent strain of the worm's bacterial food (Escherichia coli (E. coli) OP50) during early adulthood. Use of a microfluidic chip based on a thin glass coverslip substrate al ...

ROYAL SOC CHEMISTRY2023