Publications by Pascal Jourdain

Image-Based Marker-Free Screening of GABA(A) Agonists, Antagonists, and Modulators

Gerardo Turcatti, Pierre Marquet, Damiano Banfi, Pascal Jourdain, Benjamin Rappaz, Fabien Kuttler

The ionotropic GABA(A) receptors represent the main target for different groups of widely used drugs having hypnotic and anxiolytic effects. So far, most approaches used to assess GABA activity involve invasive low -throughput electrophysiological techniqu ...

2019

Mapping of real-time morphological changes in the neuronal cytoskeleton with label-free wide-field second-harmonic imaging: a case study of nocodazole

Pascal Jourdain, Sylvie Roke, Carlos Alberto Macias Romero, Marie Eve Pascale Didier, Claire Jacqueline Léa Teulon

We demonstrate the use of wide-field high-throughput second-harmonic (SH) microscopy for investigating cytoskeletal morphological changes on the single-cell level. The method allows for real-time, in vitro, label-free measurements of cytoskeletal changes t ...

SPIE-Intl Soc Optical Eng2019

Membrane water for probing neuronal membrane potentials and ionic fluxes at the single cell level

Pierre Magistretti, Pascal Jourdain, Sylvie Roke, Marie Eve Pascale Didier, Orly Bagunu Tarun

Neurons communicate through electrochemical signaling within a complex network. These signals are composed of changes in membrane potentials and are traditionally measured with the aid of (toxic) fluorescent labels or invasive electrical probes. Here, we d ...

Nature Publishing Group2018

Dual action of L-Lactate on the activity of NR2B-containing NMDA receptors: from potentiation to neuroprotection

Pierre Marquet, Pierre Magistretti, Pascal Jourdain, Igor Allaman, Kaspar Rothenfusser

L-Lactate is a positive modulator of NMDAR-mediated signaling resulting in plasticity gene induction and memory consolidation. However, L-Lactate is also able to protect neurons against excito-toxic NMDAR activity, an indication of a mitigating action of L ...

Nature Research2018

Synergistic interaction of L-Lactate and Glycine to potentiate NMDA receptor activity: a new form of meta-potentiation?

Pierre Marquet, Pierre Magistretti, Pascal Jourdain, Igor Allaman, Kaspar Rothenfusser

Wiley2017

Quantitative phase-digital holographic microscopy: a new imaging modality to identify original cellular biomarkers of diseases

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Pascal Jourdain, Benjamin Rappaz, Kaspar Rothenfusser

Quantitative phase microscopy (QPM) has recently emerged as a powerful label-free technique in the field of living cell imaging allowing to non-invasively measure with a nanometric axial sensitivity cell structure and dynamics. Since the phase retardation ...

Spie-Int Soc Optical Engineering2016

L-Lactate protects neurons against excitotoxicity: implication of an ATP-mediated signaling cascade

Pierre Marquet, Pierre Magistretti, Pascal Jourdain, Igor Allaman, Kaspar Rothenfusser

Converging experimental data indicate a neuroprotective action of L-Lactate. Using Digital Holographic Microscopy, we observe that transient application of glutamate (100 μM; 2 min) elicits a NMDA-dependent death in 65% of mouse cortical neurons in culture ...

Nature Research2016

Astrocyte swelling in response to neural activation: role of cotransporters

Pierre Marquet, Pierre Magistretti, Pascal Jourdain, Daniel Boss, Kaspar Rothenfusser

Wiley-Blackwell2015

High throughput second harmonic imaging for label-free biological applications

Pierre Marquet, Pierre Magistretti, Pascal Jourdain, Aleksandra Radenovic, Sylvie Roke, Carlos Alberto Macias Romero, Marie Eve Pascale Didier, Vitalijs Zubkovs, Orly Bagunu Tarun

Second harmonic generation (SHG) is inherently sensitive to the absence of spatial centrosymmetry, which can render it intrinsically sensitive to interfacial processes, chemical changes and electrochemical responses. Here, we seek to improve the imaging th ...

Optical Society of America2014

The human CFTR protein expressed in CHO cells activates aquaporin-3 in a cAMP-dependent pathway: study by digital holographic microscopy

Pierre Marquet, Sylvain Eric Lengacher, Pierre Magistretti, Pascal Jourdain

The transmembrane water movements during cellular processes and their relationship to ionic channel activity remain largely unknown. As an example, in epithelial cells it was proposed that the movement of water could be directly linked to cystic fibrosis t ...

Company of Biologists2014

Lactate promotes plasticity gene expression by potentiating NMDA signaling in neurons

Pierre Magistretti, Pascal Jourdain, Igor Allaman, Jean-Marie Petit, Gabriele Grenningloh, Evelyne Ruchti, Jiangyan Yang

L-lactate is a product of aerobic glycolysis that can be used by neurons as an energy substrate. Here we report that in neurons L-lactate stimulates the expression of synaptic plasticity-related genes such as Arc, c-Fos, and Zif268 through a mechanism invo ...

National Academy of Sciences2014

Lactate Modifies Neuronal Excitability Through Both Nmda And Katp Receptors: Importance For Plasticity Genes Expression

Pierre Magistretti, Pascal Jourdain, Igor Allaman, Jean-Marie Petit, Gabriele Grenningloh, Evelyne Ruchti, Jiangyan Yang

Wiley-Blackwell2013

Marker-free phase nanoscopy

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Nicolas Pavillon, Pascal Jourdain, Daniel Boss, Yann Jérôme Michel Pascal Cotte, Muhammed Fatih Toy

We introduce a microscopic method that determines quantitative optical properties beyond the optical diffraction limit and allows direct imaging of unstained living biological specimens. In established holographic microscopy, complex fields are measured us ...

Nature Publishing Group2013

Lactate As A Signaling Molecule For The Regulation Of Plasticity-Related Genes Expression

Pierre Magistretti, Pascal Jourdain, Igor Allaman, Jean-Marie Petit, Gabriele Grenningloh, Evelyne Ruchti, Jiangyan Yang

Wiley-Blackwell2013

Lactate as a signaling molecule for the regulation of plasticity-related genes expression

Pierre Magistretti, Pascal Jourdain, Igor Allaman, Jean-Marie Petit, Gabriele Grenningloh, Evelyne Ruchti, Jiangyan Yang

Wiley-Blackwell2013

Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and solute flux by digital holographic microscopy

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Pascal Jourdain, Daniel Boss

A dual-wavelength digital holographic microscope to measure absolute volume of living cells is proposed. The optical setup allows us to reconstruct two quantitative phase contrast images at two different wavelengths from a single hologram acquisition. When ...

SPIE Society of Photo-optical Instrumentation Engineers2013

Simultaneous Optical Recording in Multiple Cells by Digital Holographic Microscopy of Chloride Current Associated to Activation of the Ligand-Gated Chloride Channel GABA(A) Receptor

Christian Depeursinge, Pierre Marquet, Pascal Jourdain, Daniel Boss, Benjamin Rappaz, Corinne Moratal

Chloride channels represent a group of targets for major clinical indications. However, molecular screening for chloride channel modulators has proven to be difficult and time-consuming as approaches essentially rely on the use of fluorescent dyes or invas ...

Public Library of Science2012

Early Cell Death Detection with Digital Holographic Microscopy

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Nicolas Pavillon, Pascal Jourdain, Corinne Moratal

Background: Digital holography provides a non-invasive measurement of the quantitative phase shifts induced by cells in culture, which can be related to cell volume changes. It has been shown previously that regulation of cell volume, in particular as it r ...

Public Library of Science2012

What role in cerebral activity?

Pierre Marquet, Pierre Magistretti, Pascal Jourdain

2012

Determination of Transmembrane Water Fluxes in Neurons Elicited by Glutamate Ionotropic Receptors and by the Co-transporters KCC2 and NKCC1: A Digital Holographic Microscopy Study

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Nicolas Pavillon, Pascal Jourdain, Daniel Boss, Benjamin Rappaz, Corinne Moratal

Digital holographic microscopy (DHM) is a noninvasive optical imaging technique that provides quantitative phase images of living cells. In a recent study, we showed that the quantitative monitoring of the phase signal by DHM was a simple label-free method ...

Society for Neuroscience2011