Publications by Hüseyin Cumhur Tekin

Dose-response curve of a microfluidic magnetic bead-based surface coverage sandwich assay

Thomas Lehnert, Martinus Gijs, Hüseyin Cumhur Tekin, Matteo Cornaglia, Raphaël Etienne Jean Trouillon

Magnetic micro-and nanoparticles ('magnetic beads') have been used to advantage in many microfluidic devices for sensitive antigen (Ag) detection. Today, assays that use as read-out of the signal the number count of immobilized beads on a surface for quant ...

Elsevier2015

Microfluidic chip for monitoring Ca2+ transport through a confluent layer of intestinal cells

Martinus Gijs, Paolo Silacci, Guy Vergères, Hüseyin Cumhur Tekin, Josias Basil Wacker, Christine Ruffert

The absorption of dietary calcium through the intestinal barrier is essential for maintaining health in general and especially of the bone system. We propose a microfluidic model that studies free calcium (Ca2+) transport through a confluent monolayer of C ...

Royal Soc Chemistry2014

Magnetic Particle-Scanning for Ultrasensitive Immunodetection On-Chip

Thomas Lehnert, Martinus Gijs, Hüseyin Cumhur Tekin, Matteo Cornaglia, Raphaël Etienne Jean Trouillon

We describe the concept of magnetic particle-scanning for on-chip detection of biomolecules: a magnetic particle, carrying a low number of antigens (Ag's) (down to a single molecule), is transported by hydrodynamic forces and is subjected to successive sto ...

American Chemical Society2014

A random-walk based model to explain ultrasensitive magnetic bead-based immunoassays

Thomas Lehnert, Martinus Gijs, Hüseyin Cumhur Tekin, Matteo Cornaglia, Raphaël Etienne Jean Trouillon

We introduce a new modeling approach for understanding the fundamental principle of bead-based surface coverage immunoassays that rely on the specific interaction between surface-bound “small” magnetic beads (1 μm in diameter) and a flow of “large” magneti ...

2014

Fine-tuning of magnetic and microfluidic viscous forces for specific magnetic bead-based immunocomplex formation

Thomas Lehnert, Martinus Gijs, Hüseyin Cumhur Tekin, Matteo Cornaglia

We investigate the working principle of a novel type of microfluidic sandwich immunoassay, as used for the detection of biomarkers. The heterogeneous assay is based on the specific interactions between an array of functionalized superparamagnetic beads and ...

American Institute of Physics2013

Self-assembled melamine microlens arrays for immunofluorescence enhancement

Abdeljalil Sayah, Martinus Gijs, Hüseyin Cumhur Tekin, Hui Yang

Self-assembled dielectric microlenses are used for focusing of the fluorescent signal emitted from a surface-based immunoassay, performed on silane micropatterns as assay substrates, to enhance the detection signal. In our model system, a fluorescent immun ...

2013

Ultrasensitive protein detection: a case for microfluidic magnetic bead-based assays

Martinus Gijs, Hüseyin Cumhur Tekin

Royal Society of Chemistry2013

Attomolar protein detection using a magnetic bead surface coverage assay

Martinus Gijs, Hüseyin Cumhur Tekin, Matteo Cornaglia

We demonstrate a microfluidic method for ultra-sensitive protein detection in serum. First, ‘large’ (2.8 μm) antibody-functionalized magnetic beads specifically capture antigen from a serum matrix under active microfluidic mixing. Subsequently, the large b ...

Royal Society of Chemistry2013

Automated Analysis of Rare Biomarkers Using a Magnetic Bead Surface Coverage Assay Coupled with Chaotic Mixing on a Microfluidic Chip

Hüseyin Cumhur Tekin

Extremely sensitive protein detection is crucial for early diagnosis and monitoring of diseases. Present day’s limit of detection for an antibody-antigen recognition-based immunoassay in serum without having to use special target antigen amplification tech ...

EPFL2012

Microchannel-guided capture antibody patterning on beads for multiple protein detection arrays

Martinus Gijs, Venkataragavalu Sivagnanam, Hüseyin Cumhur Tekin, Sébastien Vaneberg

We present the rapid fabrication of custom protein micro-arrays, in less than 35 minutes, on the surface of immobilized beads using microchannel-guided flows driven by a single syringe pump. The commercially available beads provide robust micro-array surfa ...

2011

Microfluidic device for analysis of protein biomarkers using magnetic bead surface coverage detection

Martinus Gijs, Hüseyin Cumhur Tekin, Cynthia Scherz

We present a microfluidic device for on-chip analysis of low-concentration protein biomarkers. A new detection method, based on the magnetic capture of proteins via superparamagnetic beads and counting the surface coverage of the latter is presented. The p ...

2011

Chaotic mixing using source-sink microfluidic flows in a PDMS chip

Abdeljalil Sayah, Martinus Gijs, Caroline Vandevyver, Venkataragavalu Sivagnanam, Hüseyin Cumhur Tekin, Ata Tuna Ciftlik

We present an active fixed-volume mixer based on the creation of multiple source–sink microfluidic flows in a polydimethylsiloxane (PDMS) chip without the need of external or internal pumps. To do so, four different pressure-controlled actuation chambers a ...

Springer-Verlag2011

Active microfluidic mixer using virtual source-sink pairs for DNA purification

Martinus Gijs, Caroline Vandevyver, Hüseyin Cumhur Tekin

We present an active mixer based on the creation of virtual source-sink pairs in a polydimethylsiloxane (PDMS) chip. Perfect mixing is achieved in only a sub-second time interval for a compact 5 μL mixing volume. This excellent performance is achieved than ...

2010

A fixed-volume microfluidic mixer realized using multilayer PDMS technology

Martinus Gijs, Hüseyin Cumhur Tekin

We present leakage-free valves and an efficient fixed-volume mixer fabricated using a four-layer soft lithography technique in polydimethylsiloxane (PDMS). Mixing is achieved with a 95% efficiency in a few seconds by creating circular flow patterns inside ...

Elsevier Science, Reg Sales Off, Customer Support Dept, 655 Ave Of The Americas, New York, Ny 10010 Usa2009

Double gate movable body Micro-Electro-Mechanical FET as hysteretic switch: Application to data transmission systems

Maher Kayal, Mihai Adrian Ionescu, Cédric Meinen, Daniel Grogg, Hüseyin Cumhur Tekin, Dimitrios Tsamados

This paper reports on the fabrication, experimental characterization and data transmission application of a double-gate movable body FET. As its name suggests, the proposed movable-body Micro-Electro-Mechanical FET (MB-MEMFET) is a hybrid MEMS-semiconducto ...

2008

Laterally vibrating-body double gate MOSFET with improved signal detection

Mihai Adrian Ionescu, Daniel Grogg, Marco Mazza, Nicoleta-Diana Ciressan, Hüseyin Cumhur Tekin, Dimitrios Tsamados

Laterally vibrating-body double-gate MOSFET that shows a +30 dB signal increase compared to an identical resonator operating with pure capacitive detection is reported. The double-gate MOSFET is fabricated on SOI substrate with silicon film thickness of 1. ...

2008

Nano-gap high quality factor thin film SOI MEM resonators

Mihai Adrian Ionescu, Daniel Grogg, Marco Mazza, Nicoleta-Diana Ciressan, Hüseyin Cumhur Tekin, Dimitrios Tsamados

A 1.25 m thin SOI micro-electro-mechanical (MEM) resonator with a quality factor of 100’000 at 24.6 MHz is demonstrated. A nanogap fabrication process allows the fabrication of < 200 nm gaps, allowing for low polarization voltages. A motional resistance of ...

2008

DNA sensor using interdigitated microelectrodes and micro/nanoparticles

Martinus Gijs, Daniel Berdat, Hüseyin Cumhur Tekin

2008

Micropatterning of streptavidin-coated magnetic microbeads in-situ in a microfluidic channel using electrostatic self-assembly

Abdeljalil Sayah, Martinus Gijs, Venkataragavalu Sivagnanam, Hüseyin Cumhur Tekin

2007