Publications associées à Porosome

Water as a contrast agent for imaging interfacial structure and ion transport in giant vesicles

Hydrated lipid bilayer membranes and their asymmetry play a fundamental role in living cells by maintaining and regulating concentration gradients between cells, their environment, and their compartments. They achieve this not only through various channels ...

EPFL2022

Calcium-triggered fusion of lipid membranes is enabled by amphiphilic nanoparticles

Francesco Stellacci, Ye Yang, Ahmet Bekdemir, Zekiye Pelin Güven

Lipid membrane fusion is an essential process for a number of critical biological functions. The overall process is thermodynamically favorable but faces multiple kinetic barriers along the way. Inspired by nature's engineered proteins such as SNAP recepto ...

National Academy of Sciences2020

Calcium-Mediated Liposome Fusion to Engineer Giant Lipid Vesicles with Cytosolic Proteins and Reconstituted Mammalian Proteins

Leo Scheller

Giant unilamellar lipid vesicles (GUVs) are widely used as model membrane systems and provide an excellent basis to construct artificial cells. To construct more sophisticated artificial cells, proteins-in particular membrane proteins-need to be incorporat ...

2020

Generation of Native, Untagged Huntingtin Exon1 Monomer and Fibrils Using a SUMO Fusion Strategy

Hilal Lashuel, Jonathan Jean-Pierre Ricci, Anass Chiki, Andreas Reif

Huntington’s Disease (HD) is an inherited fatal neurodegenerative disease caused by a CAG expansion (36) in the first exon of the HD gene, resulting in the expression of the Huntingtin protein (Htt) or N-terminal fragments thereof with an expanded polygl ...

2018

Nano Secondary Ion Mass Spectrometry Imaging of Dopamine Distribution Across Nanometer Vesicles

Anders Meibom, Anna Larsson

We report an approach to spatially resolve the content across nanometer neuroendocrine vesicles in nerve-like cells by correlating super high-resolution mass spectrometry imaging, NanoSIMS, with transmission electron microscopy (TEM). Furthermore, intracel ...

American Chemical Society2016

Semisynthetic and in Vitro Phosphorylation of Alpha-Synuclein at Y39 Promotes Functional Partly Helical Membrane-Bound States Resembling Those Induced by PD Mutations

Hilal Lashuel, Bruno Claude Daniel Fauvet, Anne-Laure Mahul Mellier, Carole Desobry, Farah El Turk, Ana Jovicic

Alpha-synuclein is a presynaptic protein of poorly understood function that is linked to both genetic and sporadic forms of Parkinson's disease. We have proposed that alpha-synuclein may function specifically at synaptic vesicles docked at the plasma membr ...

Amer Chemical Soc2016

Molecular mechanisms governing Ca2+ regulation of evoked and spontaneous release

Ralf Schneggenburger

The relationship between transmitter release evoked by action potentials and spontaneous release has fascinated neuroscientists for half a century, and separate biological roles for spontaneous release are emerging. Nevertheless, separate functions for spo ...

2015

An Exclusion Zone for Ca2+ Channels around Docked Vesicles Explains Release Control by Multiple Channels at a CNS Synapse

Henry Markram, Ralf Schneggenburger, Olexiy Kochubey, Felix Schürmann, Yunyun Han, Daniel Keller, Zsolt Norbert Babai

The spatial arrangement of Ca2+ channels and vesicles remains unknown for most CNS synapses, despite of the crucial importance of this geometrical parameter for the Ca2+ control of transmitter release. At a large model synapse, the calyx of Held, transmitt ...

Public Library Science2015

Purification of centrosomes from mammalian cell lines

Paul Guichard

Centrosomes act as the main microtubule-organizing centre of animal cells and play critical roles in the cell, such as mitotic spindle organization, cell polarity, and motility. They are composed of two barrel-shaped structures, the centrioles, surrounded ...

Elsevier Academic Press Inc2015

Lipid tail protrusions mediate the insertion of nanoparticles into model cell membranes

Francesco Stellacci, Kislon Voitchovsky, Maria Ricci, Paulo Henrique Jacob Silva, Javier Reguera Gomez

Recent work has demonstrated that charged gold nanoparticles (AuNPs) protected by an amphiphilic organic monolayer can spontaneously insert into the core of lipid bilayers to minimize the exposure of hydrophobic surface area to water. However, the kinetic ...

Nature Publishing Group2014

An Alien Divalent Ion Reveals a Major Role for Ca2+ Buffering in Controlling Slow Transmitter Release

Ralf Schneggenburger, Olexiy Kochubey, Daniel Keller, Zsolt Norbert Babai

Ca2+-dependent transmitter release occurs in a fast and in a slow phase, but the differential roles of Ca2+ buffers and Ca2+ sensors in shaping release kinetics are still controversial. Replacing extracellular Ca2+ by Sr2+ causes decreased fast release but ...

2014

Role of Protein Kinase C and Munc18-1 in a Presynaptic Form of Plasticity, Post-tetanic Potentiation

Özgür Genç

Neurons are arranged in networks in which they communicate between each other by the means of synapses. Synaptic transmission can undergo activity-dependent and short-lived changes in strength, known as short-term synaptic plasticity. Short-term plasticity ...

EPFL2013

Ca2+ channels and transmitter release at the active zone

Ralf Schneggenburger, Olexiy Kochubey, Yunyun Han

Ca2+-dependent transmitter release is the most important signaling mechanism for fast information transfer between neurons. Transmitter release takes places at highly specialized active zones with sub-micrometer dimension, which contain the molecular machi ...

Elsevier2012

RIM Determines Ca²+ Channel Density and Vesicle Docking at the Presynaptic Active Zone

Yunyun Han

Fast neurotransmitter release is essential for neuron-neuron communication and is initiated by the opening of voltage-gated Ca2+ channels close to docked vesicles at the presynaptic active zone. The high concentration of Ca2+ channels at the active zone is ...

EPFL2011

Solid-state nanopore array membranes patterned by electron beam lithography, nanosphere lithography and aluminum anodization

Jürgen Brugger, Andreea Veronica Savu, Songmei Wu, Oscar Vazquez Mena, Harry Heinzelmann, Vaida Auzelyte, Mona Julia Katharina Klein, Nicolas Blondiaux, Raphaël Pugin, Franck Montagne

We present the fabrication of thin membranes with dense arrays of nanometer and submicrometer pore arrays by the integration of standard micromachining with three pore patterning techniques: electron beam lithography (EBL), nanosphere lithography (NSL) and ...

2011

Solid-state Nanopore Array Membranes Patterned by Electron Beam Lithography, Nanosphere Lithography and Aluminum Anodization

Jürgen Brugger, Andreea Veronica Savu, Oscar Vazquez Mena, Songmei Wu, Mona Julia Katharina Klein, Vaida Auzelyte, Nicolas Blondiaux, Franck Montagne

We present the fabrication of thin membranes with dense arrays of nanometer and submicrometer pore arrays by the integration of standard micromachining with three pore patterning techniques: electron beam lithography (EBL), nanosphere lithography (NSL) and ...

Research Publishing Services2011

Regulation of transmitter release by Ca2+ and synaptotagmin: insights from a large CNS synapse

Ralf Schneggenburger, Olexiy Kochubey

Transmitter release at synapses is driven by elevated intracellular Ca2+ concentration (Ca2+) near the sites of vesicle fusion. Ca2+ signals of profoundly different amplitude and kinetics drive the phasic release component during a presynaptic ac ...

2011