Publications associées à Chapelet

High-Density Immobilization of TCEP on Silica Beads for Efficient Disulfide Reduction and Thiol Alkylation in Peptides

Tris-(2-carboxyethyl)phosphine (TCEP) linked to agarose beads is widely used for reducing disulfide bridges in proteins and peptides. The immobilization of TCEP on beads allows efficient removal after reduction to prevent its reaction with alkylating reage ...

2023

Whirling instability of an eccentric coated fibre

François Gallaire, Edouard Boujo, Shahab Eghbali, Ludovic Pierre Gustave Keiser

We study a gravity-driven viscous flow coating a vertical cylindrical fibre. The destabilisation of a draining liquid column into a downward moving train of beads has been linked to the conjunction of the Rayleigh-Plateau and Kapitza instabilities in the l ...

Cambridge University Press2022

Dielectrophoresis detection device

Carlotta Guiducci, Pierre-Emmanuel Marie Thiriet

A dielectrophoretic detection device comprising a chip, with a flow channel having at least one inlet and one outlet, and at least a detection area configured to detect analytes trapped on functionalised beads flowing within said flow channel, a first and ...

2021

Preparation of Highly Porous Metal-Organic Framework Beads for Metal Extraction from Liquid Streams

Wendy Lee Queen, Emad Oveisi, Daniel Teav Sun, Shuliang Yang, Li Peng, Olga Trukhina, Olga Syzgantseva, Ilia Kochetygov, Anita Justin, Hassan Abedini, Guanchu Lu

Metal-organic frameworks (MOFs) offer great promise in a variety of gas- and liquid-phase separations. However, the excellent performance on the lab scale hardly translates into pilot- or industrial-scale applications due to the microcrystalline nature of ...

AMER CHEMICAL SOC2020

Permeability of textile fabrics with spherical inclusions

Véronique Michaud, Baris Çaglar

We investigated the effect of rigid spherical inclusions such as microcapsules and fillers on the permeability of fabrics by using glass beads as model inclusions. Beads with a range of diameters (40-800 mu m) and volume fractions (2.5-10%) were sieved bet ...

Elsevier2017

Dose-response curve of a microfluidic magnetic bead-based surface coverage sandwich assay

Martinus Gijs, Thomas Lehnert, Matteo Cornaglia, Raphaël Etienne Jean Trouillon, Hüseyin Cumhur Tekin

Magnetic micro-and nanoparticles ('magnetic beads') have been used to advantage in many microfluidic devices for sensitive antigen (Ag) detection. Today, assays that use as read-out of the signal the number count of immobilized beads on a surface for quant ...

Elsevier2015

Neutral solar photo-Fenton degradation of 4-nitrophenol on iron-enriched hybrid montmorillonite-alginate beads (Fe-MABs)

César Pulgarin, Salvatore Barreca

Hybrid montmorillonite-alginate beads (MABs) were prepared by the ion-gelation method from alginate and montmorillonite clay suspension dropped in a calcium chloride solution. Similarly, iron-enriched beads (Fe-MABs) were prepared using iron-exchanged mont ...

Elsevier Science Sa2014

Attomolar protein detection using a magnetic bead surface coverage assay

Martinus Gijs, Matteo Cornaglia, Hüseyin Cumhur Tekin

We demonstrate a microfluidic method for ultra-sensitive protein detection in serum. First, ‘large’ (2.8 μm) antibody-functionalized magnetic beads specifically capture antigen from a serum matrix under active microfluidic mixing. Subsequently, the large b ...

Royal Society of Chemistry2013

Automated Analysis of Rare Biomarkers Using a Magnetic Bead Surface Coverage Assay Coupled with Chaotic Mixing on a Microfluidic Chip

Hüseyin Cumhur Tekin

Extremely sensitive protein detection is crucial for early diagnosis and monitoring of diseases. Present day’s limit of detection for an antibody-antigen recognition-based immunoassay in serum without having to use special target antigen amplification tech ...

EPFL2012

Simultaneous sample washing and concentration using a “trapping-and-releasing” mechanism of magnetic beads on a microfluidic chip

Martinus Gijs

Simultaneous washing and concentration of functionalized magnetic beads in a complex sample solution was demonstrated by applying a rotational magnetic actuation system to a microfluidic chip under continuous flow conditions. The rotation of periodically a ...

2011

Microchannel-guided capture antibody patterning on beads for multiple protein detection arrays

Martinus Gijs, Hüseyin Cumhur Tekin, Venkataragavalu Sivagnanam, Sébastien Vaneberg

We present the rapid fabrication of custom protein micro-arrays, in less than 35 minutes, on the surface of immobilized beads using microchannel-guided flows driven by a single syringe pump. The commercially available beads provide robust micro-array surfa ...

2011

Nanoporous SiN membranes patterned by wafer-scale nanosphere lithography

Jürgen Brugger, Andreea Veronica Savu, Oscar Vazquez Mena, Harry Heinzelmann, Mona Julia Katharina Klein, Raphaël Pugin, Franck Montagne

Nanoporous SiN membranes are interesting for a number of applications, e.g. stenciling, filtration, or tissue engineering [1-3]. The patterning of the pores requires a high resolution lithographic tool. Focused ion beam milling or e-beam lithography fulfil ...

2010

Study of Spatio-Temporal Immunofluorescence on Bead Patterns in a Microfluidic Channel

Martinus Gijs, Venkataragavalu Sivagnanam, Hui Yang

We performed a direct immunoassay inside a microfluidic channel on patterned streptavidin-coated beads, which captured fluorescently-labeled biotin target molecules from a continuous flow. We arranged the beads in a dot array at the bottom of the channel a ...

American Institute of Physics2010

On-chip immunoassay using electrostatic assembly of streptavidin-coated bead micropatterns

Martinus Gijs, Caroline Vandevyver, Venkataragavalu Sivagnanam

We propose an original concept for a sandwich immunoassay that is completely performed on-chip using streptavidin-coated beads as substrate. The latter are electrostatically self-assembled on aminosilane micropatterns at the bottom of a microfluidic channe ...

2009

On-chip immunoassay using electrostatic assembly of streptavidin-coated bead micropatterns

Martinus Gijs, Caroline Vandevyver, Venkataragavalu Sivagnanam, Hui Yang, Bo Song

We propose an original concept for a sandwich immunoassay that is completely performed on-chip using streptavidin-coated beads as substrate. The latter are electrostatically self-assembled on aminosilane micropatterns at the bottom of a microfluidic channe ...

2009

Large eddy simulation of pollen transport in the atmospheric boundary layer

Marc Parlange, Charles Vivant Ignacio Meneveau

This paper presents a framework to simulate pollen dispersal by the wind based on the large eddy simulation(LES) technique. Important phenomena such as the pollen emission by the plants and the ground deposition are parameterized by the lower boundary cond ...

2009

Sandwich immunoassay on a microfluidic chip using patterns of electrostatically self-assembled streptavidin-coated beads

Martinus Gijs, Caroline Vandevyver, Frédéric Lacharme, Venkataragavalu Sivagnanam

In this paper, we demonstrate a bead-based microfluidic immunoassay using self-assembled streptavidin-coated bead micropatterns. The beads are assembled on the microfluidic channel surface using electrostatic forces between the negatively-charged streptavi ...

Elsevier2009

Coupled tomography and distinct-element-method approach to exploring the granular media microstructure in a jamming hourglass

Giorgio Margaritondo, Michaël Unser, Thomas Liebling, Daniel Sage, Lionel Pournin, Michel Tsukahara, Slobodan Mitrovic, Marco Ramaioli, Vincent Gajdosik

We describe an approach for exploring microscopic properties of granular media that couples x-ray microtomography and distinct-element-method (DEM) simulations through image analysis. We illustrate it via the study of the intriguing phenomenon of instant a ...

APS2008

Bead-based single protein micro-array realized through electrostatic self-assembly of carboxylated beads

Martinus Gijs, Abdeljalil Sayah, Venkataragavalu Sivagnanam

We report here a simple method, based on electrostatic self-assembly, for realizing bead-based single protein micro-arrays. We generate positively charged surface micro-patterns on a glass substrate and use them as template for adsorbing negatively-charged ...

2008

Bead-based protein microarrays realized through electrostatic self-assembly of carboxylated beads

Martinus Gijs, Abdeljalil Sayah, Venkataragavalu Sivagnanam

We report here a simple method, based on electrostatic self-assembly, for realizing bead-based single protein microarrays. We generate positively-charged surface micro-patterns on a glass substrate and used them as the template for adsorbing negatively-cha ...

2007