Publications associées à Isoforme

Precision Targeting of pten-Null Triple-Negative Breast Tumors Guided by Electrophilic Metabolite Sensing

Yimon Aye, Xuyu Liu, Lingxi Wang, Chaosheng Luo

Off-target effects continue to impede disease interventions, particularly when targeting a specific protein within a family of similar proteins, such as kinase isoforms that play tumor-subtype-specific roles in cancers. Exploiting the specific electrophili ...

AMER CHEMICAL SOC2020

Enteric infection induces Lark-mediated intron retention at the 5 ' end of Drosophila genes

Bart Deplancke, Maroun Bou Sleiman, Michael Vincent Frochaux, Dani Osman, Tommaso Andreani

Background RNA splicing is a key post-transcriptional mechanism that generates protein diversity and contributes to the fine-tuning of gene expression, which may facilitate adaptation to environmental challenges. Here, we employ a systems approach to study ...

2020

The mRNA-Binding Protein HuR Is a Kinetically-Privileged Electrophile Sensor

Yimon Aye, Jesse Poganik, Yi Zhao, Alexandra Krina Van Hall-Beauvais

The key mRNA-binding proteins HuR and AUF1 are reported stress sensors in mammals. Intrigued by recent reports of sensitivity of these proteins to the electrophilic lipid prostaglandin A2 and other redox signals, we here examined their sensing abilities to ...

2020

Cytoplasmic Ubiquitin-Specific Protease 19 (USP19) Modulates Aggregation of Polyglutamine-Expanded Ataxin-3 and Huntingtin through the HSP90 Chaperone

Françoise Gisou van der Goot Grunberg

Ubiquitin-specific protease 19 (USP19) is one of the deubiquitinating enzymes (DUBs) involved in regulating the ubiquitination status of substrate proteins. There are two major isoforms of USP19 with distinct C-termini; the USP19_a isoform has a transmembr ...

Public Library of Science2016

alpha-Synuclein-induced dopaminergic neurodegeneration in a rat model of Parkinson's disease occurs independent of ATP13A2 (PARK9)

Bernard Schneider, Elpida Tsika, Liliane Glauser, Alessandra Musso, Guillaume Daniel, Darren Moore, Aris Nikolaos Fiser

Mutations in the ATP13A2 (PARK9) gene cause early-onset, autosomal recessive Parkinson's disease (PD) and Kufor-Rakeb syndrome. ATP13A2 mRNA is spliced into three distinct isoforms encoding a P5-type ATPase involved in regulating heavy metal transport acro ...

Academic Press Inc Elsevier Science2015

Transcriptomic characterization of the enzymatic antioxidants FeSOD, MnSOD, APX and KatG in the dinoflagellate genus Symbiodinium

Thomas Krüger

Background The diversity of the symbiotic dinoflagellate Symbiodinium sp., as assessed by genetic markers, is well established. To what extent this diversity is reflected on the amino acid level of functional genes such as enzymatic antioxidants that play ...

BioMed Central2015

A chemostat array enables the spatio-temporal analysis of the yeast proteome

Michaël Unser, Felix Naef, Sebastian Maerkl, Nicolas Dénervaud, Ricard Delgado Gonzalo, Johannes Becker, Arun Stephen Rajkumar

Observing cellular responses to perturbations is central to generating and testing hypotheses in biology. We developed a massively parallel microchemostat array capable of growing and observing 1,152 yeast-GFP strains on the single-cell level with 20 min t ...

National Academy of Sciences2013

A transcript perspective on evolution

Bernard Moret, Yann Christinat

Alternative splicing is now recognized as a major mechanism for transcriptome and proteome diversity in higher eukaryotes, yet its evolution is poorly understood. Most studies focus on the evolution of exons and introns at the gene level, while only few co ...

Ieee Computer Soc2013

Vulnerability of conditional NCAM-deficient mice to develop stress-induced behavioral alterations

Maria del Carmen Sandi Perez, Reto Bisaz

Previous studies in rodents showed that chronic stress induces structural and functional alterations in several brain regions, including shrinkage of the hippocampus and the prefrontal cortex, which are accompanied by cognitive and emotional disturbances. ...

2012

Characterization of semisynthetic and naturally Nα-acetylated α-synuclein in vitro and in intact cells: implications for aggregation and cellular properties of α-synuclein

Hilal Lashuel, Bruno Claude Daniel Fauvet, Mohamed Bilal Fares

N-terminal acetylation is a very common post-translational modification, although its role in regulating protein physical properties and function remains poorly understood. α-Synuclein (α-syn), a protein that has been linked to the pathogenesis of Parkinso ...

Amer Soc Biochemistry Molecular Biology Inc2012

Role of NCAM in emotion and learning

Maria del Carmen Sandi Perez, Kamila Markram, Reto Bisaz, Lisa Conboy

NCAM is an abundant cell adhesion molecule known to be important during development. Together with its posttranslational modification consisting of the addition of the polysaccharide polysialic acid (PSA), NCAM has been classically implicated in the regula ...

2010

Design and synthesis of acetamido tri- and tetra-hydroxyazepanes: Potent and selective beta-N-acetylhexosaminidase inhibitors

Pierre Vogel, Claudia Bello

A series of seven-membered iminosugars bearing an acetamido group beta- or gamma- to the endocyclic nitrogen have been synthesized via simple transformations of previously described polysubstituted azepanes. These tetra- and trihydroxylated acetamido azepa ...

2009

Molecular dynamics calculations suggest a conduction mechanism for the M2 proton channel from influenza A virus

Matteo Dal Peraro

The M2 protein of the influenza A virus is activated by low endosomal pH and performs the essential function of proton transfer into the viral interior. The resulting decrease in pH within the virion is essential for the uncoating and further replication o ...

2009

Overexpression of a mutant form of TGFBI/BIGH3 induces retinal degeneration in transgenic mice

Purpose: Despite ubiquitous expression of the keratoepithelin (KE) protein encoded by the transforming growth factor beta induced/beta induced gene human clone 3 (TGFBI/BIGH3) gene, corneal dystrophies are restricted to the cornea, and no other tissues are ...

2008

Triggering of Bcl-2-related pathway is associated with apoptosis of photoreceptors in Rpe65(-/-) mouse model of Leber's Congenital Amaurosis

Mutations in RPE65 protein is characterized by the loss of photoreceptors, although the molecular pathways triggering retinal cell death remain largely unresolved. The role of the Bcl-2 family of proteins in retinal degeneration is still controversial. How ...

2008

An engineered protein tag for multi-protein labeling in living cells

Kai Johnsson, Christian Heinis, Alexandre Juillerat

The visualization of complex cellular processes involving multiple proteins requires the use of spectroscopically distinguishable fluorescent reporters. We have previously introduced the SNAP-tag as a general tool for the specific labeling of SNAP-tag fusi ...

2008