Publications de Alexandre Juillerat

Labelling of Fusion Proteins with Synthetic Probes

The invention relates to new proteins called alkylcytosine transferases (ACTs) derived from O6-alkylguanine-DNA alkyltransferase, and to substrates for ACTs specifically transferring a label to these ACTs and to fusion proteins comprising these. The substr ...

2013

Labelling of fusion proteins with synthetic probes

Kai Johnsson, Alexandre Juillerat

The invention relates to new proteins called alkylcytosine transferases (ACTs) derived from O-alkylguanine-DNA alkyltransferase, and to substrates for ACTs specifically transferring a label to these ACTs and to fusion proteins comprising these. The subs ...

2008

A split-protein sensor for studying protein-protein interactions in mycobacteria

Kai Johnsson, Alexandre Juillerat

2008

An engineered protein tag for multi-protein labeling in living cells

Kai Johnsson, Alexandre Juillerat, Christian Heinis

The visualization of complex cellular processes involving multiple proteins requires the use of spectroscopically distinguishable fluorescent reporters. We have previously introduced the SNAP-tag as a general tool for the specific labeling of SNAP-tag fusi ...

2008

Mutants of o6-alkylguanine-dna alkyltransferase

Kai Johnsson, Christopher Chidley, Antje Keppler, Alexandre Juillerat, Thomas Gronemeyer, Christian Heinis

The invention relates to AGT mutants showing, when compared to the wild type human AGT, two or more advantageous properties selected from (a) reduced DNA interaction (b) localisation of the expressed protein in eukaryotic cells that is no longer restricted ...

2006

Directed evolution of O6-alkylguanine-DNA alkyltransferase for applications in protein labeling

Kai Johnsson, Christopher Chidley, Alexandre Juillerat, Thomas Gronemeyer, Christian Heinis

The specific reaction of O6-alkylguanine-DNA alkyltransferase (AGT) with O6-benzylguanine (BG) derivatives allows for a specific labeling of AGT fusion proteins with chemically diverse compounds in living cells and in vitro. The efficiency of the labeling ...

Oxford University Press2006

Engineering substrate specificity of O6-alkylguanine-DNA alkyltransferase for specific protein labeling in living cells

Hugues Jaccard, Kai Johnsson, Alexandre Juillerat, India Christina Sielaff, Béatrice Kunz, Christian Heinis

Fusion proteins of human O6-alkylguanine-DNA alkyltransferase (AGT) can be specifically labeled with a wide variety of synthetic probes in mammalian cells; this makes them an attractive tool for studying protein function. However, to avoid undesired labeli ...

2005

Engineering of the human O6-alkylguanine DNA alkyltransferase

Alexandre Juillerat

Recently, a new method for the specific covalent labeling of fusion proteins in vitro and in living cells has been developed. This method is based on the unusual mechanism of a DNA repair protein: the O6-alkylguanine DNA alkyltransferase (AGT). In addition ...

EPFL2005