Publication

On-Chip Sandwich Immunoassay in an Integrated Magneto-Optical CMOS Microsystem

Edoardo Charbon, Martinus Gijs, Ulrike Lehmann, Emile Dupont
2008
Conference Papers

Résumé

We report on an innovative approach to on-chip detection of single fluorescently-labeled magnetic micro-particles by means of Single Photon Avalanche Diode (SPAD) arrays. Our system enables particle separation through magnetic actuation and highly sensitive, high signal-to-noise-ratio detection with integrated SPADs. We will present the capability of our system to detect and quantitatively distinguish fluorescently labeled particles. This represents a first step towards a complete lab-on-a-chip system for specific antibody detection. The selection of magnetic micro-particles as the mobile substrate in a bio-assay is motivated by their growing impact on the lab-on-a-chip research area, fueled by their ability of contactless manipulation and a handling that is independent from biological processes.

Source officielle

https://infoscience.epfl.ch/entities/publication/2b644627-d5f5-4423-b1f3-9559aecc24db

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Edoardo Charbon, Martinus Gijs, Ulrike Lehmann, Emile Dupont
2008
Conference Papers

Résumé

Source officielle

https://infoscience.epfl.ch/entities/publication/2b644627-d5f5-4423-b1f3-9559aecc24db

À propos de ce résultat

Concepts associés (12)

Laboratoire sur puce

Un laboratoire sur puce est un dispositif intégré rassemblant, sur un substrat miniaturisé, une ou plusieurs fonctions de laboratoire. L'analyse du vivant regroupe trois des quatre raisons majeures ayant entraîné le développement de la microfluidique ; elle représente par conséquent une large part des applications. On considère généralement que le premier dispositif microfluidique d'analyse est celui développé par Terry et al. ; ceux-ci réalisent en 1979 un système miniaturisé d'analyse de gaz par chromatographie sur un substrat de silicium.

Organ-on-a-chip

An organ-on-a-chip (OOC) is a multi-channel 3-D microfluidic cell culture, integrated circuit (chip) that simulates the activities, mechanics and physiological response of an entire organ or an organ system. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context.

Droplet-based microfluidics

Droplet-based microfluidics manipulate discrete volumes of fluids in immiscible phases with low Reynolds number and laminar flow regimes. Interest in droplet-based microfluidics systems has been growing substantially in past decades. Microdroplets offer the feasibility of handling miniature volumes (μl to fl) of fluids conveniently, provide better mixing, encapsulation, sorting, sensing and are suitable for high throughput experiments.

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