Publications de Isabelle Barde

The questions you should ask yourself for efficient CRISPR/Cas9 genome editing

Springer2016

Release of human cytomegalovirus from latency by a KAP1/TRIM28 phosphorylation switch

Didier Trono, Isabelle Barde, Adamantia Kapopoulou, Benjamin Rauwel, Marco Cassano, Suk Min Jang

Human cytomegalovirus (HCMV) is a highly prevalent pathogen that induces life-long infections notably through the establishment of latency in hematopoietic stem cells (HSC). Bouts of reactivation are normally controlled by the immune system, but can be fat ...

2015

Modulation of mitophagy and use thereof

Didier Trono, Isabelle Barde

The present invention relates to an agent or composition comprising at least one of: (i) a micro-RNA targeting a mitophagy-associated gene or a variant thereof, (ii) a vector comprising a nucleic acid encoding said micro-RNA or variant thereof, (iii) an in ...

2014

Control of mitophagie by microRNAs - A key step of erythropoiesis

Didier Trono, Isabelle Barde, Sonia Verp, Andrea Corsinotti, Jiri Vanicek, Sandra Eloise Kjeldsen, Adamantia Kapopoulou, Ray Marcel Marin Florez, Benjamin Rauwel

Edp Sciences S A2014

A KRAB/KAP1-miRNA Cascade Regulates Erythropoiesis Through Stage-Specific Control of Mitophagy

Didier Trono, Isabelle Barde, Sonia Verp, Andrea Corsinotti, Jiri Vanicek, Sandra Eloise Kjeldsen, Adamantia Kapopoulou, Ray Marcel Marin Florez, Benjamin Rauwel

During hematopoiesis, lineage- and stage-specific transcription factors work in concert with chromatin modifiers to direct the differentiation of all blood cells. Here, we explored the role of KRAB-containing zinc finger proteins (KRAB-ZFPs) and their cofa ...

American Association for the Advancement of Science2013

Efficient sperm cryopreservation and recovery of mouse lines from various backgrounds

Isabelle Barde, Sonia Verp, Sandra Eloise Kjeldsen, Xavier Warot, Michelle Blom

Springer2013

Measuring In Vivo Protein Half-Life

Kai Johnsson, Christian Heinis, Didier Trono, Isabelle Barde, Sonia Verp, Karolina Bojkowska, Francesca Santoni de Sio, Sandra Eloise Kjeldsen

Protein turnover critically influences many biological functions, yet methods have been lacking to assess this parameter in vivo. Here, we demonstrate how chemical labeling of SNAP-tag fusion proteins can be exploited to measure the half-life of resident i ...

Elsevier2011

Regulation of Episomal Gene Expression by KRAB/KAP1-Mediated Histone Modifications

Patrick Aebischer, Vivianne Padrun, Christopher Towne, Didier Trono, Isabelle Barde, Sonia Verp, Anna Claire Groner

KAP1 is an essential cofactor of KRAB zinc finger proteins, a family of vertebrate-specific epigenetic repressors of largely unknown functions encoded in the hundreds by the mouse and human genomes. So far, KRAB/KAP1-mediated gene regulation has been studi ...

American Society for Microbiology2009

KRAB can repress lentivirus proviral transcription independently of integration site

Didier Trono, Isabelle Barde

The KRAB transcriptional repressor domain, commonly found in zinc finger proteins, acts by inducing the formation of heterochromatin. We previously exploited this property to achieve drug-regulated transgenesis and knock down by combining doxycycline-contr ...

2006